【摘要】 目的研究两种不同基因型NS3抗原在HCV感染者血清学诊断中的应用价值。方法分别用两种不同基因型NS3单片段抗原、两种单片段抗原的混合物(MIX)作为包被抗原,对血清标本进行ELISA测定;应用反转录套式聚合酶链反应(RT-nPCR)检测血清中HCV RNA,数据以χ2检验。结果 85份抗-HCV阳性血清以不同基因型HCV NS3单片段抗原检测,阳性检出分别为52份(Ⅰ型)和50份(Ⅵ型),χ2=0.06,P>0.05;100份健康成人体检血清以此两种抗原检测均为阴性;90份抗-HCV阳性血清经MIX抗原检测,阳性检出为65份;51份抗-HCV阳性血清经RT-nPCR检测,29份阳性。结论 HCV不同基因型NS3编码区抗原片段有不同的抗原反应性,在HCV感染者的血清学诊断中将不同基因型NS3抗原混合使用可能是更好的选择。更多还原

【Abstract】 OBJECTIVE To explore the immunoreactivity of two NS3 recombinant antigens derived from different genotypes and to evaluate the application of the antigens on the serological diagnosis in patients infected with HCV.METHODS Two HCV NS3 recombinant antigens derived from HCV genotype 1 and 6 were applied in anti-HCV ELISA detection,respectively.The known anti-HCV-positive specimens and normal adult sera were tested with mixture of the two antigens.RT-nPCR based on universal primers for multiple HCV genotypes was used to detect HCV RNA.Data analysis was done by chi-square test.RESULTS 52 positive serum samples were detected out using the NS3 antigen from HCV genotype 1 and 50 samples detected out using the antigen from genotype 6,respectively,among 85 known anti-HCV-positive specimens.No difference between the two positive rates of anti-NS3 were found(χ2=0.06,P>0.05).No antigenicity was found in 100 serum samples obtained from normal adult blood donors by both the two NS3 antigens.Sixty-five of 90 patient specimens were immunoreactive to the mixed antigens and the positive rate was 72.2%.Twenty-nine of 51 patient samples were RT-nPCR positive.CONCLUSION It shows different immunoreactivity of HCV NS3 antigens between HCV genotype 1 and 6.It may be a more better choice to apply the mixture of NS3 antigens from HCV different genotypes in the serological diagnosis in patients infected with HCV.更多还原