【摘要】 目的找出铜绿假单胞菌PAEO1野生型与PDO300、PAEO1 lasI rhlI基因缺陷型、PAEO1 lasRrhlR基因缺陷型菌株之间的差异蛋白质,探讨这些蛋白质与铜绿假单胞菌生物膜形成的关系。方法应用表面增强激光解析电离飞行时间质谱(SELDI)技术和CM10蛋白质芯片检测PAEO1野生型与PDO300、PAEO1 lasI rhlI基因缺陷型、PAEO1 lasRrhlR基因缺陷型菌株的菌体蛋白,采用Biomarker Wizard软件进行分析,筛选差异蛋白质。结果PDO300菌株与PAEO1野生型菌株相比有10个蛋白质高表达(P<0.01),相对分子质量分别为4074、3817、8923、8148、3332、4521、7621、11 594、7831、13 751;有6个蛋白质低表达(P<0.01),相对分子质量分别为6199、6899、12 379、6115、6763、8576;PAEO1 lasI rhlI基因缺陷型菌株与PAEO1野生型菌株相比,有1个蛋白质低表达(P<0.05),相对分子质量为6910,PAEO1 lasRrhlR基因缺陷型菌株与PAEO1野生型菌株相比,有2个蛋白质高表达(P<0.05),相对分子质量分别为8924和8790;有2个蛋白质低表达(P<0.05),相对分子质量分别为6735和6986。结论用蛋白质芯片和SELDI技术对铜绿假单胞菌进行蛋白质组学研究,方法简便、敏感性高,可发现一系列低Mr或低丰度的差异蛋白质。更多还原

【Abstract】 OBJECTIVE To discover different proteins among Pseudomonas aeruginosa(PAE) mucoid PDO300,wide-type P.aeruginosa PAEO1,PAEO1 lasI rhlI mutants and PAEO1 lasR rhlR mutants strains.METHODS The protein mass spectra of the tropina of mucoid PDO300,wide-type PAEO1,PAEO1 lasI rhlI and PAEO1 lasR rhlR mutants strains were detected by CM10 protein chip on surface enhanced laser desorption/ionization/time of flight/mass spectrometry(SELDI),then analyzed by Biomarker Wizard software.RESULTS Sixteen different proteins were found between mucoid PDO300 and wide-type PAEO1(P<0.01).Ten proteins with relative molecular mass(Mr) 4074,3817,8923,8148,3332,4521,7621,11 594,7831 and 13 751 were up-regulated while six proteins with Mr 6199,6899,12 379,6115,6763 and 8576 were down-regulated in the tropina of PDO300.Comparing the tropina of wide-type PAEO1 and PAEO1 lasI rhlI mutants strains,there was one protein down-regulated in PAEO1 lasI rhlI mutants strain(P<0.05).It′s Mr 6910.There were four different proteins between wide-type PAEO1 and PAEO1 lasR rhlR mutants strains(P<0.05).Two proteins with Mr 8924 and 8 790 were up-regulated while two proteins with Mr 6735 and 6986 were down-regulated in the tropina of PAEO1 lasR rhlR mutants strain.CONCLUSIONS In this study,we have taken advantage of the techniques of SELDI to detect variation in protein expression among different P.aeruginosa strains.These different proteins may contribute to the phenotypic differences or have relation to quorum sensing(QS) system.更多还原