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大鼠口服棉花皮素-8-O-β-D-葡萄糖醛酸苷后尿液中代谢产物研究

Metabolites in rat urine after orally administrating gossypetin-8-O-β-D-glucuronide

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【作者】 许彤彤郭慧王邠赵玉英张庆英

【Author】 XU Tongtong, GUO Hui, WANG Bin, ZHAO Yuying, ZHANG Qingying(Department of Natural Medicines and State Key Laboratory of Natural and Biomimetic Drugs, School ofPharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China)

【机构】 北京大学医学部药学院天然药物学系及天然药物与仿生药物国家重点实验室

【摘要】 目的:研究大鼠口服棉花皮素-8-O-β-D-葡萄糖醛酸苷后尿液中的代谢产物。方法:健康大鼠口服250mg·kg-1棉花皮素-8-O-β-D-葡萄糖醛酸苷后,收集0~48h的尿样,经HPD-600大孔吸附树脂分离纯化后采用HPLC-DAD-MSn方法对尿样中的原形药物及其代谢产物进行分析及结构推测。结果:在大鼠尿液中检测到棉花皮素-8-O-β-D-葡萄糖醛酸苷的6个主要代谢产物,根据负离子模式下电喷雾质谱的裂解规律、紫外光谱特征以及与标准品对照的方法,鉴定了其中1个代谢产物的结构,推测了其他5个代谢产物的结构,其代谢过程主要是葡萄糖醛酸化、脱葡萄糖醛酸以及甲基化等。结论:本文建立的方法快速、灵敏,可用于棉花皮素-8-O-β-D-葡萄糖醛酸苷及其体内外代谢产物的研究。

【Abstract】 Objective:To investigate the metabolites in rat urine after orally administrating gossypetin-8-O-β-D-glucuronide. Method: Gossypetin-8-O-β-D-glucuronide was administrated orally at the dose of 250 mg·kg-1 to healthy rats, then the urine was collected, pre-treated appropriately and detected by HPLC-DAD-MSn. Result: In the rat urine, gossypetin-8-O-β-D-glucuronide, along with 6 metabolites, were detected, and their structures were elucidated on the basis of reference standards, UV and MSn spectra. The metabolic pathways of gossypetin-8-O-β-D-glucuronide included mainly deglycosylation, glucuronidation and methylation. Conclusion: A rapid and sensitive method for the analysis of metabolites in rat urine after orally administrating gossypetin-8-O-β-D-glucuronide was established and this will be useful for the further metabolism study of gossypetin-8-O-β-D-glucuronide.

【基金】 教育部长江学者和创新团队计划项目(985-2-063-112);国家自然科学基金项目(20872006)
  • 【文献出处】 中国中药杂志 ,China Journal of Chinese Materia Medica , 编辑部邮箱 ,2010年19期
  • 【分类号】R285.5
  • 【被引频次】1
  • 【下载频次】304
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