【摘要】 目的:以野生地黄为对象,分离和鉴定地黄内生真菌,并探讨其内生真菌与地黄连作状态之间的关系。方法:将野生地黄进行不同的移植处理,获得根部膨大状态不同的地黄根系,分离其内生真菌,分别通过棉兰染色法和18S rDNA及ITS序列分析对内生真菌进行形态学和分子鉴定,比较内生菌的差异;并建立地黄块根膨大的水培实验系统,将地黄无菌苗和内生真菌进行共培养,观察内生真菌对地黄生长的影响。结果与结论:共分离到4种内生真菌,从未膨大根系中分离出3种优势菌株Ⅰ,Ⅱ,Ⅲ,从膨大的地黄根中分离到Ⅲ和Ⅳ,经鉴定这4种真菌分别为I轮生菌Verticillium spp,Ⅱ尖孢镰刀菌Fusarium oxysporum,Ⅲ芳香镰孢菌F.redolens,Ⅳ角担菌属真菌Ceratobasidiumspp.。共培养结果表明内生真菌Ⅰ,Ⅱ,Ⅲ的过量生长不利于地黄根膨大,而Ⅳ则不影响地黄根部的膨大。更多还原

【Abstract】 The swollen root of Rehmannia glutinosa is used as one kind of important Chinese traditional medicine.The root of R.glutinosa usually swelled in rotational cropping but not in continuous cropping.The rhizosphere microorganisms of R.glutinosa under different farming condition were thought related to that.In this study,the endophytic fungi in the root of R.glutinosa growing in various soil conditions were isolated for the study of the relationship between the microorganisms and the root enlargement of their host plants.The dominant endophytes,Verticillium spp.,Fusarium oxysporum,F.redolens and Ceratobasidium spp.were identified by morphological observation and 18S rDNA and ITS sequence analysis.The preliminary investigation showed that the excessive growth of Verticillium and Fusarium genus fungi is unfavorable for the R.glutinosa root swelling,but Ceratobasidium fungi has no effects on the root enlargement.更多还原