【摘要】 目的:探讨夏枯草愈伤组织诱导和植株再生条件。方法:采用正交试验法研究不同外植体、蔗糖、植物生长物质及其配比对夏枯草愈伤组织诱导和分化的影响。结果和结论:以叶片为外植体诱导愈伤效果最好,茎段次之,叶柄不能诱导出愈伤组织;6-BA是影响夏枯草愈伤组织形成的主要因素;诱导愈伤组织的适宜培养基为MS+6-BA 3.0 mg.L-1+NAA 0.1mg.L-1+2,4-D 0.5 mg.L-1+蔗糖3%;宜于愈伤组织分化的培养基为1/2 MS+6-BA 3.25 mg.L-1+NAA1.25 mg.L-1+蔗糖2%;最佳生根培养基:MS+IBA 1.0 mg.L-1+蔗糖3%。更多还原

【Abstract】 Objective: To study the conditions of callus induction and plantlet regeneration of Prunella vulgaris.Method: By using the orthogonal experiment design,different explants,sucrose,plant growth substances and their ratio for callus induction and differentiation were optimized.Result and Conclusion: The inductivity of leaves was the highest,followed by stems,but callus of the leafstalks could not be induced.The main factor of affecting the formation of callus was 6-BA.The optimal medium for callus induction was MS+6-BA 3.0 mg·L-1 + NAA 0.1 mg·L-1 + 2,4-D 0.5 mg·L-1+ sucrose 3%.The optimal medium for callus differentiation was 1/2 MS+6-BA 3.25 mg·L-1 + NAA 1.25 mg·L-1 + sucrose 2%.The optimal medium for rooting was MS+IBA 1.0 mg·L-1+sucrose 3%.更多还原