【摘要】 目的:探讨外源一氧化氮（nitric oxide,NO）对胃癌AGS细胞生长及c-jun和c-fos表达的影响。方法:应用MTT法评价NO供体硝普钠（sodium nitroprusside,SNP）,SNP的同源类似物铁氰化钠Na₃Fe（CN）₆和一氧化氮合酶（nitric oxide synthase,NOS）抑制剂N-硝基-L-精氨酸甲酯（N-nitro-L-arginine methylester,L-NAME）对胃癌细胞生长的抑制作用,RT-PCR和免疫印迹检测c-jun和c-fos的基因和蛋白表达。结果:SNP剂量依赖性的抑制AGS细胞的生长,其IC₅₀值为2.13mmol/L。1mmol/L SNP处理12、24、48h后,细胞生长抑制率分别为（16.12±0.85）%、（20.33±1.31）%、（22.06±2.03）%（P<0.05）,NOS抑制剂L-NAME预处理明显减弱了SNP对癌细胞的生长抑制效应,Na₃Fe（CN）₆对胃癌细胞生长无抑制。与对照组比较,SNP使c-jun和c-fos的mRNA和蛋白表达降低,NOS抑制剂L-NAME预处理逆转了SNP的这种作用,Na₃Fe（CN）₆对c-jun和c-fos的mRNA和蛋白表达无影响。结论:NO可诱导胃癌细胞c-jun和c-fos表达降低,抑制癌细胞生长。更多还原

【Abstract】 Objective: To investigate the effect of exogenous nitric oxide on the growth and the expressions of c-jun and c-fos in gastric cancer cell line AGS.Methods: The AGS cells were treated with SNP,Na₃Fe（CN）₆ and L-NAME.The growth inhibition of cancer cells was determined by MTT assay.The changes of mRNA expressions of c-jun and c-fos were examined by RT-PCR.The protein expressions of c-jun and c-fos were analyzed by Western blot.Results: SNP inhibited AGS cell growth in a dose-dependent manner with a 24 hour IC50 value of 2.13 mmol/L.When the AGS cells were treated with 1 mmol/L SNP for 12,24 and 48 hours,the growth inhibition rates were （16.12±0.85）%,（20.33±1.31）% and （22.06± 2.03）%,respectively （P<0.05）.L-NAME reversed the effect of SNP in inhibiting the growth of AGS cells.Na₃Fe（CN）₆ had a very small effect on the growth inhibition of AGS cells.Compared with the control group,SNP reduced the gene and protein expressions of c-jun and c-fos in gastric cancer cell line AGS.Such decreases were reversed by pretreatment with L-NAME.Na₃Fe（CN）₆ had little effect on the gene and protein expressions of c-fos and c-jun.Conclusion: Nitric oxide may work to inhibit gastric cancer cell line AGS growth by decreasing the expression of c-fos and c-jun.更多还原