【摘要】 目的:探讨结外B细胞淋巴瘤中MALT1基因(mRNA)的表达与MALT和弥漫大B细胞淋巴瘤发生发展的关系,及其对淋巴瘤临床病理特征和预后的影响。方法:用HE和免疫组化染色筛选出本院确诊的MALT和结外弥漫大B细胞淋巴瘤106例,检查肿瘤组织Bcl-2和Ki-67的表达,用RT-PCR检测肿瘤中的MALT1基因mRNA水平,收集临床病理资料并随访。结果:MALT1 mRNA的表达:在DLBCL与MALT淋巴瘤组、淋巴结累及组和无累及组、临床分期Ⅲ～Ⅳ组和Ⅰ～Ⅱ组间均有统计学差异(P<0.05),在Ki-67≥30%与<30%组间未见统计学差异(P>0.05),而Bcl-2阳性组却高于阴性组(P<0.05);MALT1mRNA表达与不表达病例之间患者生存状况有明显差异(P<0.05),表达组生存状况较差。结论:MALT1基因在MALT淋巴瘤与DLBCL中有不同程度的表达,MALT1基因活化NF-κB后可能通过调控Bcl-2使细胞凋亡抑制而导致淋巴瘤发生,MALT1mRNA表达与B细胞淋巴瘤的类型、淋巴结累及、临床分期有关,与患者的生存状况有关,MALT1可能是一个MALT和弥漫大B细胞淋巴瘤预后不良的相关因子。更多还原

【Abstract】 Objective:To investigate the relationship between MALT1 mRNA expression in extranodal B cell lymphoma and the pathogenesis of MALToma and DLBCL, and to explore the effect of MALT1 mRNA expression on the clinicopathologic features and survival of lymphoma patients. Methods:H&E and immunohistochemical staining were applied to observe the expression of Bcl-2 and Ki-67 in 106 samples of extranodal B cell lymphoma. The mRNA of MALT1 was detected by RT-PCR. Clinicopathological data were reviewed. Follow-up and statistical analysis were performed. Results:Expression of MALT1 mRNA was statistically different between the cases with lymph node metastasis and those without lymph node mestastasis, and between staging Ⅰ-Ⅱ cases and stage Ⅲ-Ⅳ cases, The expression of MALT1 mRNA in cases with positive expression of MALToma, DLBCL, and Bcl-2 was higher than in those without expression of the three indices (P<0.05). However, no significant difference was found in MALT1 mRNA expression between cases with Ki-67≥30% and those with Ki-67<30% (P>0.05). MALT1 mRNA-positive cases showed a worse survival status than MALT1 mRNA-negative cases (P< 0.05). Conclusion:Expression of MALT1 gene is different between MALToma and DLBCL. Inhibition of apoptosis by Bcl-2 overexpression caused by activation of NF-κB may lead to the pathogenesis of MALToma and DLBCL. The expression of MALT1 mRNA is associated with the types of lymphoma, involvement of lymph node, stage of lymphoma, and patient survival. The expression of MALT1 gene may be associated with poor prognosis of MALToma and DLBCL.更多还原