【摘要】 目的:探讨CD40和COX-2在食管鳞状细胞癌组织中的表达情况、临床病理意义及其与肿瘤血管生成的关系。方法:采用免疫组织化学方法研究79例食管癌和28例正常食管黏膜上皮组织中CD40和COX-2的表达情况,根据CD34表达计算食管癌组织平均微血管密度(MVD);分析CD40、COX-2的表达与食管癌发生部位、肿瘤大小、分化程度、MVD及淋巴结转移的关系。同时采用免疫细胞化学染色及Western Blot方法检测食管癌Eca109细胞和原代培养的正常食管上皮细胞CD40和COX-2在蛋白水平上表达的差异。结果:免疫组化结果表明,食管癌组织中CD40和COX-2的阳性表达率均明显高于正常食管鳞状上皮(54.43%vs10.71%,69.62%vs17.86%,P均<0.05)。CD40在食管癌中的表达与淋巴结转移有关,伴有淋巴结转移者CD40阳性表达率明显高于无淋巴结转移者(70.37%vs46.15%,P<0.05),但CD40表达与其他临床病理特征无明显相关。COX-2在食管癌中的阳性表达与肿瘤大小、部位、分化程度、有无淋巴结转移等均无明显相关(P>0.05)。CD40与COX-2在食管癌组织中表达明显相关(P<0.05),相关系数(Φ)为0.446。食管癌组织中MVD明显高于正常食管组织(25.02±5.52vs12.09±4.55,P<0.05)。CD40和COX-2阳性表达组食管癌组织MVD明显高于阴性表达组(26.37±6.02vs22.58±5.25,P<0.05)。体外研究结果表明,食管癌Eca109细胞CD40和COX-2表达均高于正常食管上皮细胞(P<0.05)。结论:CD40表达与食管鳞状细胞癌的发生、发展密切相关,CD40可能通过影响COX-2的表达促进食管癌组织微血管生成。更多还原

【Abstract】 Objective:To explore the putative role of CD40 and COX-2 expression in human esophageal squamous cell carcinoma (ESCC) and to analyze their possible relationship with angiogenesis in ESCC.Methods: The expression of CD40 and COX-2 was detected in 79 ESCC and 28 normal esophageal epithelial tissue samples with immunohistochemical staining.The microvessel density by CD34 was determined and the clinicopathological significance of CD40 and COX-2 expression in ESCC was analyzed.In addition,the expression of CD40 and COX-2 in esophageal squamous cell carcinoma cell line Eca109 and primary cultured normal esophageal epithelial cells in vivo was comparatively studied with immunocytochemical staining and Western blot.Results:Compared with that in normal epithelial tissues,the expression of CD40 and COX-2 in ESCC was significantly higher (54.43% vs 10.71%; 69.62% vs 17.86%; respectively,P<0.05).The positive ex- pression rate of CD40 in ESCC cases with lymph node metastasis was significantly higher than that in those without lymph node metastasis (70.37% vs 46.15%,P<0.05).No correlation was found between CD40 expression and patient age,sex,tumor location,size and differentiation of tumors.No clinicopathological significance of COX-2 expression in ESCC was found.There was a positive correlation between CD40 expression and COX-2 expression in esophageal squamous cell carcinoma (P<0.05,φ=0A46).The mean MVD value in ESCC was significantly higher than that in normal esophageal tissue (25.02±5.52 vs 12.09±4.55,P<0.05). MVD value in ESCC was closely correlated with lymph node metastasis.The mean MVD value in ESCC cases with positive CD40 and COX-2 expression was higher than that in those with negative CD40 and COX-2 expression (26.37±6.02 vs 22.58±5.25,P<0.05).Western blot results showed that CD40 and COX-2 expression in Eca109 was higher than that in cultured normal esophageal epithelial cells (P<0.05).Conclusion:The expression of CD40 is involved in the carcinogenesis and progression of esophageal squamous cell carcinoma. CD40 may play an important role in the angiogenesis of ESCC through promoting COX-2 expression.更多还原