【摘要】 目的:探讨缝隙连接与经络现象之间的联系。方法:分别采用1-庚醇阻断山羊腧穴缝隙连接及小鼠缝隙连接蛋白43(Cx43)基因敲除方法,用新型组织氧分压传感针检测山羊及小鼠膀胱经经穴("肝俞""大肠俞"等)及旁开非经穴处的氧分压。结果:①山羊膀胱经经穴的组织氧分压高于其旁开对照点,两者间差异有统计学意义(P<0.01);②注射1-庚醇后,山羊膀胱经经穴的氧分压较未注射前及注射生理盐水均明显升高(均P<0.01);③缝隙连接蛋白Cx43野生型(WT)小鼠膀胱经经穴的组织氧分压明显高于其旁开非经穴点,差异有统计学意义(均P<0.01);缝隙连接蛋白Cx43杂合型(HT)小鼠上述各穴位点的组织氧分压也高于其旁开对照点,但差异无统计学意义(均P>0.05);④WT小鼠各穴位点的组织氧分压均明显高于HT小鼠(均P<0.05),而各非经穴点的氧分压两组间比较差异无统计学意义(均P>0.05)。结论:缝隙连接在针刺信号转导过程中具有重要作用。更多还原

【Abstract】 Objective To explore the relation between gap junction and meridian phenomenon.Methods The oxy-gen partial pressure in acupoints ["肝俞"(BL 18)"大肠俞"(BL 25) etc.] and in their corresponding non-acupoints of the Bladder Meridian was observed with the needle-type tissue oxygen tension sensor in the gap junction blocking goats by 1-Heptanol injection and the Connexin 43(Cx43) gene knockout mice.Results ① The oxygen partial pressure in acupoints of Bladder Meridian on goats was higher than that in non-acupoints after 1-Heptanol injection with significant differences between them(both P<0.01).② The oxygen partial pressure in acupoints of Bladder Meridian on goats increased significantly after injecting 1-Heptanol as compare with that either injecting normal saline or injecting nothing with significant differences between them(all P<0.01).③ The oxygen partial pressure in acupoints of the Bladder Meridian was significantly higher than that in the non-acupoint controls in Cx43 wild type(WT) mice(all P<0.01).In Cx43 heterozygote(HT) mice,the oxygen partial pressure between acupoints and non-acupoint controls showed no significant differences(all P>0.05).④ In acupoints,the oxygen partial pressure in Cx43 WT mice was significantly higher than that in Cx43 HT mice(all P<0.05),while in the corresponding non-acupoints,this difference had no statistically significant(all P>0.05).Conclusion Gap junction maybe the essential factor in signal transduction of acupuncture.更多还原