【摘要】 目的:观察逆灸"关元"穴对自然更年期大鼠体内与应激相关的激素及蛋白、基因的影响,探讨其保护作用机制。方法:选健康SD雌性大鼠共128只。其中4月龄16只,作为年轻对照组;另将112只已出现动情周期紊乱的10月龄SD雌性大鼠随机分为7组:10、12、14、16月龄对照组;12、14、16月龄逆灸组,每组16只。采用放射免疫、免疫组化、原位杂交方法,观察在10月龄逆灸"关元"穴(2次/周,连续8周)对随后12、14、16月龄大鼠下丘脑不同核团热休克蛋白70(HSP70)、HSP70mRNA、促肾上腺皮质激素释放激素(CRH)、β-内啡肽(β-EP)及血浆促肾上腺皮质激素(ACTH)的影响。结果:各自然月龄组室旁核、弓状核、视上核HSP70及mRNA表达呈总体升高趋势。CRH、β-EP各自然月龄组呈波动性下降趋势,ACTH总体升高。逆灸组较同月龄对照组比较:室旁核HSP70在14月龄降低,其mRNA在12、16月龄升高(均P<0.05);弓状核HSP70在12、14月龄,HSP70mRNA在12月龄均明显升高(P<0.05,P<0.01);视上核HSP70在14月龄,HSP70mRNA在12、16月龄均明显下降(均P<0.05)。CRH、ACTH在14月龄均显著降低(P<0.05,P<0.01);β-EP含量在12月龄明显上升(P<0.05)。结论:逆灸"关元"穴对随后自然更年期大鼠下丘脑内应激蛋白、基因及体内应激激素的调节作用,可能是该疗法实现保护作用的途径之一。更多还原

【Abstract】 Objective To investigate the effect of preventive moxibustion at the acupoint of "Guanyuan" (CV 4) on the stress-related hormones,proteins and genes in natural menopausal rats,and explore its protective mechanism.Methods One hundred and twenty eight healthy female SD rats were used in this study,in which 16 rats aged 4 months were used as normal control group,the rest 10-month-old rats with disorder of estrus cycle were randomly divided into 7 groups,including 4 control groups at the age of 10,12,14 and 16 months,and 3 preventive moxibustion groups at the age of 12,14 and 16 months,16 cases in each group.The 10-month-old rats were treated with preventive moxibustion at the acupoint of "Guanyuan" (CV 4),twice a week for 8 weeks consistently,then observed on the following 12,14 and 16 months.Its effects on the level of heat shock protein 70 (HSP 70),heat shock protein 70 mRNA (HSP 70 mRNA),corticotropin-releasing hormone (CRH),β-endorphin (β-EP),adrenocorticotrophic hormone (ACTH) in the different nuclei of hypothalamus were examined with radio-immunity,immunohistochemistry or in situ hybridization methods.Results In the control groups,the expression of HSP 70 and HSP 70 mRNA was increased in paraventricular,arcuate and supraoptic nucleus,the level of CRH,β-EP was showed with declining trend in volatility,while the content of ACTH was increased.In comparison to the same-aged control groups,the preventive moxibustion groups were showed that the expression of HSP 70 in the 14-month-old group was lower and the expression of HSP 70 mRNA in the 12 and 16-month-old group was significantly higher in the paraventricular nucleus (all P<0.05),the expression of HSP 70 in the 12 and 14-month-old groups and HSP 70 mRNA in the 12-month-old group was significantly increased in the arcuate nucleus (P<0.05,P<0.01),while the expression of HSP 70 in the 14-month-old group and HSP 70 mRNA in the 12 and 16-month-old group was significantly decreased in the supraoptic nucleus (all P<0.05),the content of CRH and ACTH decreased significantly in 14-month group (P<0.05,P<0.01),but the content of β-EP obviously went up in the 12-month-old group (P<0.05).Conclusion Preventive moxibustion at the acupoint of "Guanyuan" (CV 4) can regulate the expression of stress proteins,genes and hormones in the hypothalamus for the natural menopausal rats,which might be playing a protective role.更多还原