【摘要】 目的:建立银杏叶充液胶囊中黄酮和萜类内酯的含量测定方法。方法:采用高效液相色谱法,色谱柱为依利特C18(150mm×4.6mm,5μm);流动相分别为甲醇-0.8%磷酸溶液(47∶53)、四氢呋喃-甲醇-水(3∶7∶17);流速分别为0.8,0.5mL.min-1;紫外检测波长为360nm;示差检测器;柱温均为45℃。结果:3种黄酮和4种内酯均能达到较好的分离效果,线性关系良好,平均回收率分别为98.14%,99.18%,98.81%,99.07%,99.33%,99.40%,98.95%,RSD分别为1.09%,1.16%,1.43%,1.62%,1.76%,1.72%,1.68%。结论:该方法灵敏、准确、专属性强,可作为银杏叶充液胶囊的质量控制的有效方法。更多还原

【Abstract】 OBJECTIVE To establish a determination method for liquid-filled capsules of Ginkgo biloba flavonoids and terpene lactone.METHODS The high-performance liquid chromatography,column: According to Bartlett C18(150 nm×4.6 nm,5 μm);mobile phase were methanol-0.8% phosphoric acid(47∶53),tetrahydrofuran-methanol-water(3∶7∶17);flow rate,respectively 0.8 mL·min-1,0.5 mL·min-1;detectors were UV detector(detection wavelength of 360 nm),differential detector;column temperatures were 45 ℃.RESULTS The three kinds of flavonoids and four kinds of lactone were able to achieve a better separation efficiency,good linearity,average recovery were 98.14%,99.18%,98.81%,99.07%,99.33%,99.40%,98.95%,RSD respectively,1.09%,1.16%,1.43%,1.62%,1.76%,1.72%,1.68%.CONCLUSION This method is sensitive,accurate,specific,which can be used as an effective method of quality control of liquid-filled capsules Ginkgo biloba.更多还原