【摘要】 目的建立HPLC法测定射干抗病毒注射液中绿原酸和咖啡酸的含量。方法采用Hypersil ODS柱,0.01mol.L-1磷酸二氢钠溶液(1%磷酸溶液调节pH值至3.9)-甲醇(85∶15)为流动相,流速:1.0mL.min-1,检测波长:323nm,柱温:30℃。结果绿原酸和咖啡酸浓度分别在0.40~200mg.L-1(r=0.9996)、0.16~160mg.L-1(r=0.9986)范围内与峰面积呈良好线性关系,平均加样回收率分别为99.8%,100.7%。结论本法简便、准确、重复性好,可用于射干抗病毒注射液中绿原酸和咖啡酸含量的同时测定。更多还原

【Abstract】 Objective A HPLC method for determination of chlorogenic acid and caffeic acid in Shegan Kangbingdu Injection was established.Methods A Hypersil ODS column was used with 0.01mol·L-1 sodium dihydrogen phosphate(adjust pH value to 3.9 with 1% phosphate acid solution) and methal(85∶15) as mobile phase at flow rate of 1.0mL·min-1.The detection wavelength was 323nm and the column temperature was 30℃.Results The linear ranges of chlorogenic acid and caffeic acid were 0.40～200mg·L-1(r=0.9996) and 0.16～160mg·L-1(r=0.9986),respectively.The average recoveries were 99.8% and 100.7%,respectively.Conclusion The method is simple,accurate and reproducible for determination of chlorogenic acid and caffeic acid in Shegan Kangbingdu Injection simutaneously.更多还原