【摘要】 为研究口蹄疫病毒(FMDV)致弱前后基因组变化的关系,本研究根据GenBank上发表的口蹄疫病毒全基因序列数,设计了8对引物,采用RT-PCR方法分别扩增出FMDVR株及其致弱毒株(R304)编码区的8段基因,将各片段克隆至pMD18-T载体,并进行序列测定。比较和分析编码区各个基因(LP、VP4、VP2、VP3、VP1、2A、2B、2C、3A、3B、3C、3D)的核苷酸序列和氨基酸序列的变异。结果表明,R株和R304株基因组区全长均为6966nt,编码2322个氨基酸,致弱后共有110个核苷酸发生变化,编码氨基酸有32个发生变化;其中3A基因的氨基酸序列变化最大,其次为LP和VP1,而2A和2C的氨基酸未发生变异。该研究对口蹄疫病毒的抗原和毒力研究有重要的参考价值。更多还原

【Abstract】 In this study,eight DNA fragments covered the full elngth genome of Foot-and-mouth disease virus R strain and its attenuated variant were amplified by RT-PCR using specific primers. Each fragment was cloned to pMD18-T vector and sequenced. The results showed that the genome of R strain and the attenuated strain shared the same ORF of 6 966 nt which encoded for 2322 amino acid. However,there were 110 nucleotides and 32 amino acids changes in the attenuated strain,of which the 3A gene mutated most frequently,followed by the LP gene and VP1 gene ranged third. No mutations occurred in 2A gene and 2C gene.更多还原