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一株鸡源H6N1亚型禽流感病毒全基因的分子特征

Molecular characterizations of a isolated from chicken H6N1 subtype avian influenza virus

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【作者】 刘丽玲李雁冰施建忠田国彬张翼曾显营刘娣陈化兰

【Author】 LIU Li-ling1,2,LI Yan-bing1,SHI Jian-zhong1,TIAN Guo-bin1,ZHANG Yi1,ZENG Xian-ying1,LIU Di2,CHEN Hua-lan1 (1. Animal Influenza Laboratory of the Ministry of Agriculture and State Key Labotory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China; 2. Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)

【机构】 中国农业科学院哈尔滨兽医研究所农业部动物流感重点开放实验室/兽医生物技术国家重点实验室黑龙江省农业科学院

【摘要】 2008年国家禽流感参考实验室在我国禽流感流行病学调查期间分离到1株鸡源H6N1亚型禽流感病毒(AIV)A/Chicken/ZheJiang/80/2008(H6N1)(简称为CK/ZJ/80/08),为了弄清该病毒的分子特征,我们对其8个基因片段分别进行扩增和序列测定,对每个基因进行BLAST分析,找出同源性最高的毒株。利用DNAStar中的Megalign功能进行进化分析。结果表明CK/ZJ/80/08的HA裂解位点附近的氨基酸序列为QIETR↓GLF,推测可能为一株低致病力AIV。其HA基因与日本北海道的A/duck/Hokkaido/228/2003(H6N8)和黑龙江的A/mallard/Heilongjiang/131/2006(H6N2)以及香港早期分离株A/chicken/HongKong/17/77(H6N1)等处于同一分支;NA基因在颈部没有缺失,与A/duck/Tsukuba/718/2005(H1N1)、A/goose/Guangdong/1/96(H5N1)等处于同一分支;M基因与A/duck/Hokkaido/W90/2007(H10N7)高度同源(同源性为99%);NS基因与A/duck/Denmark/65047/04(H5N2)和A/goose/Guangdong/1/96(H5N1)处于同一分支。NP、PA、PB1、PB2分别与贵州和江西分离的H5N2亚型AIV的相应基因关系密切,同源性分别为98%、97%、97%、97%。由此推测CK/ZJ/80/08可能是由H6N2、H1N1、H10N7、H5N2等多个亚型病毒重组而成。

【Abstract】 The H6N1 subtype of avian influenza virus (AIV) CK/ZJ/80/08 (H6N1) was isolated from a chicken during an epidemiological survillence in 2008. In this study,the full genome of AIV CK/ZJ/80/08 was sequenced and analyzed. The results showed that the CK/ZJ/80/08 strain had a HA cleavage site of QIETR ↓ GLF,which is a characteristic structure of low pathogenesis AIV. The HA gene sequence was closely related to A/duck/Hokkaido/228/2003 (H6N8),A/mallard/Heilongjiang/ 131/2006 (H6N2) and A/chicken/HongKong/17/77 (H6N1). The NA genes of CK/ZJ/80/08,A/duck/Tsukuba/718/2005 (H1N1) and A/Goose/Guangdong/1/96 (H5N1) shared the same phylogenetic lineage. The M gene had the highest homology with that of A/duck/ Hokkaido/W90/2007 (H10N7),and NS genes of CK/ZJ/80/08,A/duck/Denmark/65047/04 (H5N2) and A/Goose/Guangdong/1/96 (H5N1) also shared same lineage. The NP,PA,PB1 and PB2 of CK/ZJ/80/08 was closely related to that of H5N2 subtype AIV isolated from Jiangxi province. These data showed that CK/ZJ/80/08 was possibly a reassortant virus derived from H6N2,H1N1,H10N7 and H5N2 subtypes AIV.

【基金】 农业部专项-动物流感防治与监测
  • 【文献出处】 中国预防兽医学报 ,Chinese Journal of Preventive Veterinary Medicine , 编辑部邮箱 ,2010年03期
  • 【分类号】S852.65
  • 【被引频次】9
  • 【下载频次】250
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