【摘要】 2008年国家禽流感参考实验室在我国禽流感流行病学调查期间分离到1株鸡源H6N1亚型禽流感病毒(AIV)A/Chicken/ZheJiang/80/2008(H6N1)(简称为CK/ZJ/80/08),为了弄清该病毒的分子特征,我们对其8个基因片段分别进行扩增和序列测定,对每个基因进行BLAST分析,找出同源性最高的毒株。利用DNAStar中的Megalign功能进行进化分析。结果表明CK/ZJ/80/08的HA裂解位点附近的氨基酸序列为QIETR↓GLF,推测可能为一株低致病力AIV。其HA基因与日本北海道的A/duck/Hokkaido/228/2003(H6N8)和黑龙江的A/mallard/Heilongjiang/131/2006(H6N2)以及香港早期分离株A/chicken/HongKong/17/77(H6N1)等处于同一分支;NA基因在颈部没有缺失,与A/duck/Tsukuba/718/2005(H1N1)、A/goose/Guangdong/1/96(H5N1)等处于同一分支;M基因与A/duck/Hokkaido/W90/2007(H10N7)高度同源(同源性为99%);NS基因与A/duck/Denmark/65047/04(H5N2)和A/goose/Guangdong/1/96(H5N1)处于同一分支。NP、PA、PB1、PB2分别与贵州和江西分离的H5N2亚型AIV的相应基因关系密切,同源性分别为98%、97%、97%、97%。由此推测CK/ZJ/80/08可能是由H6N2、H1N1、H10N7、H5N2等多个亚型病毒重组而成。更多还原

【Abstract】 The H6N1 subtype of avian influenza virus (AIV) CK/ZJ/80/08 (H6N1) was isolated from a chicken during an epidemiological survillence in 2008. In this study,the full genome of AIV CK/ZJ/80/08 was sequenced and analyzed. The results showed that the CK/ZJ/80/08 strain had a HA cleavage site of QIETR ↓ GLF,which is a characteristic structure of low pathogenesis AIV. The HA gene sequence was closely related to A/duck/Hokkaido/228/2003 (H6N8),A/mallard/Heilongjiang/ 131/2006 (H6N2) and A/chicken/HongKong/17/77 (H6N1). The NA genes of CK/ZJ/80/08,A/duck/Tsukuba/718/2005 (H1N1) and A/Goose/Guangdong/1/96 (H5N1) shared the same phylogenetic lineage. The M gene had the highest homology with that of A/duck/ Hokkaido/W90/2007 (H10N7),and NS genes of CK/ZJ/80/08,A/duck/Denmark/65047/04 (H5N2) and A/Goose/Guangdong/1/96 (H5N1) also shared same lineage. The NP,PA,PB1 and PB2 of CK/ZJ/80/08 was closely related to that of H5N2 subtype AIV isolated from Jiangxi province. These data showed that CK/ZJ/80/08 was possibly a reassortant virus derived from H6N2,H1N1,H10N7 and H5N2 subtypes AIV.更多还原