【摘要】 目的建立高效液相色谱法测定大黄中番泻苷A含量,为大黄质量标准化研究提供依据。方法采用高效液相色谱法,色谱柱为C18(200 mm×4.6 mm,5μm),乙腈∶水∶冰醋酸(60∶40∶10)为流动相,流速1.2 ml/min,检测波长340 nm。结果番泻苷A在0.5~2.5μg范围内具有良好的线性关系,r=0.999 7,回收率为96.7%。结论用高效液相色谱法测定中药大黄中番泻苷A含量快速、可靠、方便、准确。更多还原

【Abstract】 Objective To use high performance liquid chromatography(HPLC)method for determination of sennoside A in Rhubarb,establish the quality standard for Rhubarb and provide evidence for its standardization study.Methods The HPLC mehtod was applied to determine sennoside A,chromatographic column was C18(20 cm×46 cm,5 μm),mobile phase was acetonitrile-water-glacial acetic acid(60∶40∶10),flow rate was 1.2 ml/min,detection wavelength was 340 nm.Results The sennoside A had good linearity within 0.5-2.5 μg.When r was 0.999 7,the recovery rate was 96.7%.Conclusion The method is fast,reliable,convenient and accurate.更多还原