【摘要】 为分析猪戊型肝炎病毒的基因特征,设计了1对套式引物,利用RT-PCR方法克隆出了猪戊型肝炎病毒甘肃分离株swCH196的ORF3基因cDNA片段。序列测定结果表明,swCH196株的ORF3基因长345bp,编码114个氨基酸,与GenBank中登录的其他毒株的核苷酸序列同源性为83.5%～97.7%,系统发育进化树分析结果表明,该分离株为基因Ⅳ型。更多还原

【Abstract】 To analyze the characteristics of ORF3 gene of hepatitis E virus(HEV),and establish a molecular diagnostic method,apair of primers for ORF3 gene were designed according to the published cDNA sequence of HEV in GenBank.The complete cDNA fragment of ORF3 gene was amplified from HEV swCH196 strain with the primers by RT-PCR.The results showed that the ORF3 gene of swCH196 consisted of 345bp,coded 114 amino acids,and shared 83.5%to 97.7%nucleotide homology with that of the other strains submitted to GenBank.The phylogenetic analysis showed that swCH 196 strain was classified into HEV genotypeⅣ.更多还原