【摘要】 将鹅细小病毒(GPV)H1株vp1基因从pMD18T-vp1亚克隆至原核表达载体pGEX-6P-1的多克隆位点,构建了原核表达载体pGEX-6P-vp1,将其转化感受态细胞BL21(DE3)pLysS中,经IPTG诱导,SDS-PAGE分析,结果获得了108ku的融合蛋白,该融合蛋白经亲和层析纯化并经Western-blot检测。结果表明,纯化的融合蛋白可与GPV阳性血清发生特异性反应,具有良好的抗原性。更多还原

【Abstract】 A vp1 gene of goose parvovirus(GPV) H1 strain was cloned from the pMD18T-vp1 into the polycloning sites of pGEX-6P-1 vector.The recombinant prokaryotic expression plasmid was constructed,and named pGEX-6P-vp1.The recombinant plasmid was transformed into BL21(DE3) pLysS.The transformed bacteria were induced with IPTG and the expressed protein was analyzed by SDS-PAGE.The fusion protein of 108 ku was expressed.The protein purified by chromatography purification system could react with GPV positive serum specifically in a Western-blot test,indicating that the expressed protein had good antigenicity.更多还原