【摘要】 目的:探讨L-谷氨酰胺(L-Gln)对次声暴露下大鼠海马细胞凋亡率和坏死率的影响。方法:将60只雄性SD大鼠随机分为正常对照组、次声组、次声+药物组、药物→次声组,分别将次声组、次声+药物组、药物→次声组暴露于16Hz、130dB次声环境中,正常对照组也置于次声舱内,但期间不给予次声作用。经7d相应处理后测试各组大鼠海马细胞凋亡率和坏死率的变化。结果:与正常对照组大鼠相比,次声组海马细胞凋亡率和坏死率显著升高(P<0.01);与次声组相比,次声+药物组、药物→次声组,海马细胞凋亡率和坏死率显著降低(P<0.01);与药物→次声组相比,次声+药物组海马细胞凋亡率和坏死率降低,但差异无显著性意义(P>0.05)。结论:次声(16Hz/130dB,2h/d,共7d)作用可引起大鼠海马细胞凋亡及坏死,导致海马细胞损伤;L-Gln能够部分有效预防和保护次声对大鼠海马细胞损伤的作用。更多还原

【Abstract】 Objective:To study the effects of L-glutamine(L-gln) on rat hippocampal cells’ apoptosis and necrosis rates after infrasound exposure.Method:Sixty male SD rats were randomly divided into 4 groups:normal control group,infrasound group(16Hz/130dB infrasound),infrasound + drug group(16Hz/130dB infrasound + L-Gln),drug→infrasound group(L-Gln for 7 days,and then exposed to 16Hz/130dB infrasound).No special treatment was performed on the normal control group,and the remained 3 groups were performed to L-Gln and/or infrasound treatment.7d later,hippocampus was isolated and the cell suspension was collected to detect the apoptosis and necrosis rates of hippocampal cells.Result:Compared with normal control group,apoptosis and necrosis rates of hippocampal cells in infrasound group increased significantly(P<0.01).Compared with infrasound group,the rates of apoptosis and necrosis of hippocampal cells in infrasound + drug group and drug →infrasound group decreased significantly(P <0.01).Compared with drug→infrasound group,it showed a lower rates of hippocampal cells apoptosis and necrosis in infrasound + drug group,but there was no significant difference between both groups(P>0.05).Concluson:Infrasound(16Hz/130dB,2h/d,7d) administration can induce apoptosis and necrosis of rat hippocampal cells and lead to cellullar damage in hippocampus;L-Gln can partly prevent and protect from the infrasound-induced injury on the rat hippocampus.更多还原