【摘要】 目的:动态观察经颅磁刺激仪产生的γ-氨基丁酸(GABA)和多巴胺(DA)两种特征的重复超低频磁场的连续多次经颅刺激对正常大鼠的脑内GABA、DA以及其他递质功率的影响,找出大鼠所能耐受的最长治疗时间,为此项技术应用于治疗性研究提供参考。方法:将大鼠随机分为GABA特征磁刺激组和DA特征磁刺激组,分别给予连续6次的GABA特征磁刺激和DA特征磁刺激。各组大鼠于磁刺激前、每次刺激后进行脑涨落图(EFG)测试,分别比较两组动物磁刺激前及每次磁刺激后脑内递质功率的变化。结果:GABA特征磁刺激组,从刺激前到第3次磁刺激,兴奋递质3、5-HT、Ach、兴奋递质6、NE、DA、抑制递质13的功率逐渐降低,GABA与Glu的功率则逐渐升高;第4次刺激以后,出现无规律的变化。DA特征磁刺激组,从刺激前到第2次磁刺激,全部9种递质的功率逐渐升高,第3次刺激以后,出现无规律的变化。结论:清醒大鼠对50mT的GABA特征磁场超低频经颅磁刺激耐受时长为60min,对50MT的DA特征磁场超低频经颅磁刺激耐受时长为40min。更多还原

【Abstract】 Objective:To dynamically observe the changes of neurotransmitter power of gamma aminobutyric acid (GABA),dopamine (DA) and other neurotransmitter characteristic infra-slow wave spectrum induced by GABA and DA characteristic repetitive ultra-low frequency transcranial magnetic stimulation (TMSs),in order to find the longest tolerance treatment time of rats,and provide a reference for further therapeutic intervention.Method:Sprague Dawley rats were divided into GABA group and DA group according to random method and applied with GABA and DA characteristic repetitive ultra-low frequency TMSs for 6 times respectively.The neurotransmitter characteristic infra-slow wave spectrum were recorded with encephalofluctuograph (EFG) before and after each GABA and DA characteristic ultra-low frequency TMSs respectively,the changes of neurotransmitter power of neurotransmitters pre and post stimulations of neurotransmitter characteristic repetitive ultra-low frequency TMSs in each group were compared.Result:In GABA group,from before stimulation to post the third stimulation,the infra-slow wave spectrum voltage amplitude changes of excitatory transmitter-3,5-HT,Ach,excitatory transmitter-6,NE,DA,inhibitory transmitter13 decreased gradually;while that of GABA and Glu increased gradually.After the forth stimulation irregular changes appeared.In DA group,from before stimulation to post the second stimulation,the infra-slow wave spectrum voltage amplitude changes of all the nine kinds of neurotransmitters increased gradually.After the third stimulation irregular changes appeared.Conclusion:Conscious rats can tolerate 50mT GABA characteristic repetitive ultra-low frequency TMS for 60min at most,and can tolerate 50mT DA characteristic repetitive ultra-low frequency TMS for 40min at most.更多还原