【摘要】 目的:研究大鼠脑缺血再灌注后不同时刻乳酸(Lac)和磷酸肌酸/肌酸(PCr/Cr)的波谱变化规律。方法:雄性Wistar大鼠32只,体重160—200g,随机分为正常对照组和实验组。实验组包括大脑中动脉阻塞(MCAO)模型组、假手术组、单纯梗死组,每组8只。采用1H质子波谱分析,各组均作磁共振波谱(MRS)的连续观测,观察Lac和PCr/Cr波峰的改变,以积分面积表示峰域并进行比较。结果:与正常对照组和假手术组比较,MCAO模型组缺血后出现Lac,再灌注后短暂下降继而呈上升趋势;单纯梗死组缺血出现Lac,一段时间维持后再次呈上升趋势。MCAO模型组缺血后PCr/Cr显著下降,再灌注后再次显著下降,低水平维持;单纯梗死组缺血后PCr/Cr显著下降,并在此低水平一直维持。结论:脑缺血再灌注大鼠不同时刻Lac和PCr/Cr的波谱变化可较好地反映缺血后脑组织的能量代谢变化,有无再灌注均不能改变能量缺失变化。更多还原

【Abstract】 Objective:To study the regulation of magnetic resonance spectrum (MRS) of lactate (Lac) and phosphocreatine/creatine(PCr/Cr) after cerebral ischemia/reperfusion in rats at different times.Method:Thirty-two male Wistar rats,weighting 160—200g,were randomly divided into normal control group and operation group,operation group included middle cerebral artery obstruction (MCAO) group,sham-operation group,and simple-infarction group,8 rats in each every group.Proton magnetic resonance spectroscopy was used to analysis areas under the waves of Lac and PCr/Cr and compared the data of integral areas under the waves.All the groups were under continuous observation of MRS.Result:Compared with normal control group and sham-operation group,in MCAO group the wave of Lac appeared after ischemia,descended temporarily after reperfusion and then kept the trend of rising;in simple-infarction group the wave of Lac appeared after ischemia,maintain the level at sometime and then changed the trend of rising.In MCAO group the wave of PCr/Cr descended significantly after ischemia,then descended significantly again after reperfusion,maintain the low level.In simple-infarction group the wave of PCr/Cr descended significantly after ischemia,and maintain the low level all the time.Conclusion:The variation of MRS of Lac and PCr/Cr after cerebral ischemia/reperfusion in rats can reflect the energy metabolitsm in brain tissue well.Whether reperfusion or not can not change the deficiency of energy.更多还原