【摘要】 目的探讨不同比例亚油酸(LA)/二十二碳六稀酸(DHA)对人胃癌细胞株MKN-45的抑制作用。方法采用均匀设计法初筛出LA/DHA抑制MKN-45增殖的较佳比例,采用随机区组法确定促进胃癌细胞凋亡的最佳比例;采用LA/DHA最佳比例混合脂肪酸干预MKN-45裸小鼠移植模型6周,观察裸鼠肿瘤重量、肿瘤体积变化及毒副反应情况;肿瘤组织HE染色观察肿瘤细胞形态学改变。结果经均匀设计和随机区组筛选,LA/DHA比例为1∶3时,浓度为40∶120μmol/L时,对肿瘤细胞的抑制效果最佳(P<0.05);与对照组比较,1∶3 LA/DHA混合脂肪酸高剂量组瘤体抑制率为21.97%,瘤重的抑制率为18.09%,差异有统计学意义(P<0.05);肿瘤组织病理切片显示,高剂量组和抗癌药物阳性对照组的肿瘤组织中出现不同程度坏死;高剂量组毒副反应不明显。结论LA/DHA比例为1∶3可诱导人胃癌MKN-45细胞凋亡;LA/DHA高剂量组有一定抑瘤作用,并且无明显毒副反应。更多还原

【Abstract】 Objective To explore the effects of different constituent ratio leinoleic acid(LA)/docosahexaenoic acid(DHA) on apoptosis of human gastric cancer cell line MKN-45.Methods With uniform design,the better constituent ratio for the inhibition of MKN-45 cell was scrceend.Then the best ratio was determined with randomized block design.The tumor volume,side-effect,and tumor weigh of tumor-bearing nude mice after treatment were observed.The morphological changes of tumor tissue were assessed by HE staining.Results The LA/DHA constituent ratio of 1:3 showed maximum inhibition of the tumor cells.Six weeks after high concentration treatment,the mean tumor volume decreased by 21.97% and the mean tumor weigh by 18.09% with statistical significance compared with that of control group(P<0.05).The toxicity of the treatment was not obvious.Microscopic examination showed that there were massive areas of cytoclasis and apoptosis in the tumor tissue of the high concentration group and 5-FU group.Conclusion The 1:3 constituent ratio of LA/DHA could induce the apoptosis of human gastric cancer cell line MKN-45.The high concentration of 1:3 constituent ratio treatment could also inhibit tumor in vivo without significant side-effect.更多还原