【摘要】 目的探讨血管内皮生长因子C(VEGF-C)、淋巴管内皮细胞特异性标记物D2-40与结肠直肠癌淋巴结转移的关系。方法 56例结肠直肠癌患者中有淋巴结转移30例,无淋巴结转移26例。应用免疫组织化学染色方法检测肿瘤原发灶的VEGF-C、D2-40的表达情况。结果 56例结肠直肠癌患者中有、无淋巴结转移者的VEGF-C阳性表达率分别为73.3%、15.4%,两者比较,差异有统计学意义(P<0.05);有、无淋巴结转移者D2-40标记的淋巴管密度分别为33.2±2.3、21.4±1.6,两者比较,差异有统计学意义(P<0.05)。结论结肠直肠癌患者癌组织中VEGF-C、D2-40的表达与淋巴结转移相关。更多还原

【Abstract】 Objective To study the relation between vascular endothelial growth factor C(VEGF-C),D2-40 and lymph node metastasis in colorectal cancer and its clinical significance.Methods Among 56 cases of colorectal cancer,30 cases had lymph node metastasis(metastasis group),and 26 cases had not lymph node metastasis(no metastasis group).The samples were taken in the site of primary tumor,and the preparations were processed by 4% formaldehyde fixation,paraffin embedding,sectioning and SP staining.The expressions of VEGF-C and D2-40 were detected by immunohistochemical SP method.The positive expression rate of VEGF-C and D2-40 labeled lymphatic microvessel density were calculated.Results VEGF-C positive expression rate in metastasis group was significantly higher than that in no metastasis group(73.3% vs 15.45%,P < 0.05).D2-40 labeled lymphatic microvessel density in metastasis group was significantly higher than that in no metastasis group(33.2 ± 2.3 vs 21.4 ± 1.6,P < 0.05).Conclusions The expressions of VEGF-C and D2-40 are associated with lymph node metastasis of colorectal cancer.更多还原