【摘要】 背景与目的:结直肠癌是常见的恶性肿瘤之一,在我国发病率和死亡率呈不断上升趋势。文献报道芥菜籽具有抗肿瘤的活性,本研究旨在观察在氧化偶氮甲烷(azoxymethane,AOM)诱发昆明种小鼠发生结直肠癌的过程中,通过芥菜籽(mustard seed,MS)进行饮食干预能否预防肿瘤的发生。方法:选择6周龄昆明种雌性小鼠,随机分为AOM组、AOM+5%MS干预组、AOM+10%MS干预组和正常对照组。小鼠皮下注射AOM(10mg/kg体质量),每周1次,连续3周。32周时颈椎脱臼处死小鼠,分离结直肠,用预冷的0.9%NaCl溶液冲洗肠内容物。观察记录各组小鼠有无肿瘤发生及发生数目、大小和位置,计算肿瘤发生率。结果:正常对照组小鼠未发现肿瘤,AOM模型组小鼠结直肠肿瘤发生率为86.7%,5%MS干预组及10%MS干预组小鼠结直肠肿瘤发生率分别为60.0%和41.7%,与模型组相比差异均有统计学意义(P<0.05)。AOM模型组小鼠荷瘤数为(2.2±1.2)个,而5%MS干预组和10%MS干预组小鼠荷瘤数分别为(1.1±1.1)个和(0.7±0.9)个,与AOM模型组相比差异均有统计学意义(P<0.05)。结论:通过芥菜籽饮食干预能够抑制AOM诱导的小鼠结直肠癌的发生发展,可进一步用于结直肠癌防治的研究。更多还原

【Abstract】 Background and purpose:Colorectal cancer is one of the most common cancers worldwide as well as a prevalent cause of morbidity and mortality in China.Mustard seed and its components have been reported to possess anticancer properties.The purpose of this study was to investigate the effect of a diet mixed with mustard seeds (MS) on the prevention of colorectal tumorigenesis in Kunming mice induced with azoxymethane.Methods:Sixweek-old female Kunming species mice of were randomly divided into 4 different groups:AOM alone,AOM+5% MS,AOM+10%MS,and the untreated control group.Colorectal tumorigenesis was subcutaneously induced by injecting 10 mg/kg of AOM once a week,for 3 weeks.Different doses of MS were administered in the diet during this study.Thirtytwo weeks after initiation,the mice were put down through cervical dislocation.The colon and recta of the mice were isolated and flushed with normal but ice-cold saline.The site,size,and number of colorectal tumors were recorded.The incidence of colorectal tumor in each group was determined.Results:No tumors were found in the control group.Compared to AOM-treated control group (86.7%),the occurrence of colorectal tumors was reduced to 60.0% when administered with a 5% diet of mustard seeds and to 41.7% (P<0.05) with a diet of 10% mustard seeds.Tumor burden (tumors/mouse) was 2.2±1.2 in the AOM-treated control group whereas it was reduced to 1.1±1.1 (P<0.05) in the 5% diet of mustard seeds and 0.7±0.9 (P<0.05) in the 10% diet of mustard seeds.Conclusion:The results indicate that a diet of mustard seeds is able to inhibit the development of colorectal cancer in mice induced by AOM,suggesting possible future application of MS as a chemopreventive agent for colorectal cancer.更多还原