【摘要】 目的观察星形胶质细胞短暂性缺血再灌注损伤过程中胞质和胞核的NF-E2相关因子2(Nrf2)表达变化,分析其核转位情况与细胞氧化损伤水平的相关性。方法用缺血缺氧0.5h后再复氧复注血清诱导原代培养的大鼠大脑皮质星形胶质细胞损伤,酶标仪检测各时间点细胞活性氧簇(ROS)和谷胱甘肽(GSH)水平,MTT比色法测定细胞存活率,Western blot检测胞质和胞核内Nrf2表达水平。结果随着缺血再灌注时间的延长,细胞ROS逐渐增加,GSH含量和存活率逐渐下降(P<0.05),但再灌注8h与12h比较,细胞ROS、GSH和存活率无显著性差异(P>0.05);缺血再灌注早期(再灌注0.5h),胞浆Nrf2表达减少,胞核Nrf2增多(P<0.05),继续再灌注损伤,胞浆和胞核Nrf2均减少,再灌注8h,胞浆和胞核Nrf2表达不再下降(P>0.05)。结论 Nrf2核转位与短暂性缺血再灌注损伤造成星形胶质细胞氧化应激损伤具有相关性。更多还原

【Abstract】 Objective To investigate the expression and the effect of NF-E2-related factor2 on oxidative stress in astrocytes induced by ischemia/reperfusion.Methods We used oxygen-glucose-deprivation (OGD) /reperfusion to induce the injury of primary cultured rat astrocytes,and observed the ROS level,cellular GSH content,changes of survival rate and the expression of Nrf2 in cytoplasm and nucleus respectively.Results With the prolongation of OGD/reperfusion,the cellular ROS level gradually increased,GSH content was developed and the cell viability was decreased.However,at the early stage of injury (reperfusion 0.5h),the expression of Nrf2 was decreased in cytoplasm but increased in nuclear.With the injury continuing,Nrf2 in both cytoplasm and nuclear were decreased.After 8h reperfusion,the expression of Nrf2 showed a tendency toward stabilization.Conclusion The Nrf2 nuclear translocation possesses correlation with the oxidative stress in astrocytes induced by OGD/reperfusion.更多还原