【摘要】 目的:观察重组人睫状神经营养因子(CNTF)对大鼠坐骨神经再生长的影响,并与神经生长因子(NGF)的作用进行比较。方法:随机将动物分成正常对照组、模型组、CNTF给药小剂量组(48μg/kg)、中剂量组(216μg/kg)、大剂量组(1080μg/kg)、NGF给药组(20μg/kg),每组各10只。模型组采用大鼠坐骨神经切断再缝合法造成坐骨神经损伤模型,给药组采用不同药物剂量对神经损伤部位肌肉注射给药45d后,测定神经动作电位潜伏期和传导速度。结果:与模型组相比较,CNTF各给药组神经动作电位潜伏期显著缩短(P<0.01),传导速度显著增快(P<0.01);同时,216μg/kg、1080μg/kgCNTF组和NGF组对神经动作电位潜伏期的影响无显著差别(P>0.05),但是216μg/kg、1080μg/kgCNTF组的神经动作电位传导速度比NGF组显著增快(P<0.01)。结论:CNTF对大鼠坐骨神经再生具有一定的促进作用,而且其作用可能优于NGF。更多还原

【Abstract】 AIM: To observe the effects of ciliary neurotrophic factor ( CNTF) on rat sciatic nerve regeneration,and compare the similarities and differences of its effects with that of nerve growth factor ( NGF) . METHODS: Sciatic nerve impairment model was prepared by break-restoring rat sciatic nerve. After intramuscular injection of different doses of CNTF at the damaged nerve for 45 days,the latent period and conduction velocity of action potential were determined. RESULTS: Compared with model group,the latent periods of all the CNTF treatment groups were significantly shortened ( P <0. 01) and the conduction velocity became faster significantly ( P < 0. 01) . Additionally,the effect on latent period in middle and high CNTF dosage groups is not significantly different form that in NGF treatment group ( P > 0. 05) . However,the conduction velocity in CNTF high dosage group is significantly faster than that in NGF treatment group ( P < 0. 01) . CONCLUSION: The effect of CNTF on promoting the rat sciatic nerve regeneration is better than that of NGF.更多还原