【摘要】 目的:观察姜黄素对TNF-α损伤大鼠海马神经元的功能性保护作用并探讨其机制。方法:应用离体脑片记录技术,记录大鼠海马CA1区的兴奋性突触后电位(EPSP),给予Schaffer侧支高频刺激(HFS)诱发长时程增强(LTP),观察不同药物处理组EPSP起始斜率的变化情况。结果:与对照组相比,TNF-α和NMDA(N-甲基D-天冬氨酸)对大鼠海马脑片LTP产生明显的抑制作用(P<0.05);而姜黄素可以部分拮抗TNF-α和NMDA对海马脑片LTP的抑制作用,与对照组相比无显著差异(P>0.05);TNF-α、姜黄素和NMDA对大鼠海马神经元的基础突触传递没有显著影响。结论:姜黄素对TNF-α损伤的大鼠海马神经元有功能性保护作用,其机制可能是姜黄素部分拮抗TNF-α诱导的神经元细胞膜上的NMDA受体过度激活,维持神经元的长时程增强。更多还原

【Abstract】 AIM:To observe the protective effect of curcumin on TNF-α induced neuronal damage in rat hippocampus and to explore the functional mechanism of curcumin.METHODS:The excitatory postsynaptic potential (EPSP) was recorded in CA1 pyramidal layer of rat hippocampal slices with in vitro brain slices recording techniques.High frequency stimulation was given on Schaffer branches to induce long-term potentiation (LTP).After treated with drugs,the initial slope of EPSP in each group was measured and calculated.RESULTS:Compared to control group,TNF-α and N-methyl-D-aspartate(NMDA) obviously inhibited the LTP in hippocampal slices of rat brain (P<0.05).Curcumin partly recovered the LTP,which was inhibited by TNF-α or NMDA,to near the control level (P>0.05).No effect of TNF-α,NMDA or curcumin on basal synaptic transmission in hippocampal slices was observed.CONCLUSION:Curcumin has protective effect on hippocampal neurons of rats.Curcumin can partly prevent the over-activation of NMDA receptor on neuronic membrane induced by TNF-α and maintain the long-term potentiation in neurons.更多还原