【摘要】 目的研究锰对肉鸡心肌细胞MnSOD基因的转录调节。方法将144只1日龄肉公鸡随机分为3组,分别饲喂不添加锰的基础饲粮(对照组)或添加100、200mg/kg锰(试剂级硫酸锰)的饲粮组,饲喂21d。采用全谱直读型ICP发射光谱仪分析心肌锰含量;用RT-PCR技术分析心肌细胞MnSOD基因的mRNA水平;用电泳迁移率变动分析法(EMSA)检测转录因子Sp-1和AP-2的DNA结合活性。结果心肌锰含量(P<0.002)和MnSOD mRNA水平(P<0.0001)随饲粮锰添加水平的升高而显著升高。心肌细胞转录因子Sp-1的DNA结合活性随饲粮锰添加水平的升高显著升高(P<0.0001),而其AP-2的DNA结合活性则随饲粮锰添加水平的升高显著降低(P<0.002)。结论锰可以调节控肉鸡心肌细胞MnSOD基因的转录。更多还原

【Abstract】 Objective To investigate the transcriptional regulation of heart manganese-containing superoxide dismutase(MnSOD) gene by manganese in broilers.Method One hundred forty-four one-day broilers were randomly allotted to one of three groups and fed corn-soybean meal basal diet(control) or the basal diet supplemented with 100,200 mg Mn/kg in the form of reagent-grade MnSO4·H2O for 21d.The heart Mn contents were determined by coupled plasma emission spectroscopy.Transcriptional expression levels of MnSOD gene were measured by real time-PCR.The DNA binding activities of heart transcription factor specificity protein 1(Sp-1) and activating protein 2(AP-2) were examined by electrophoretic mobility shift assay(EMSA).Results Heart Mn content(P<0.002) and MnSOD mRNA level(P<0.0001) increased markedly as dietary supplemental Mn level increased.The DNA binding activity of Sp-1 also increased as dietary Mn supplement level increased(P<0.0001),but the DNA binding activity of AP-2 decreased(P<0.002).Conclusion Manganese could transcriptionally regulate heart MnSOD gene in broilers.更多还原