【摘要】 采用开顶式同化箱(open-top chambers,OTCs)装置,设置活性碳过滤大气(CF,[O3]<10μg.kg-1)和高臭氧(O3)浓度(HO,约为80μg.kg-1)两个处理,研究开花后高浓度O3对大豆农艺性状、叶面积、叶绿素、抗氧化系统与产量的影响.结果表明:与对照(CF)同期相比,HO处理植株的叶面积和叶绿素含量显著降低(P<0.05);过氧化物酶、过氧化氢酶和超氧化物歧化酶活性极显著增强(P<0.01),但随着处理时间的延长,其活性逐渐降低;HO处理下植株叶片中可溶性蛋白质和抗坏血酸(AsA)含量降低,丙二醛含量显著升高,表明膜脂过氧化进程加快;大豆的单株干物质量、有效结荚数、籽粒数、百粒重和产量都有所降低,其中产量降低了47%,差异达极显著水平(P<0.01).更多还原

【Abstract】 By using open top chambers(OTCs),soybean plants were grown in pots,and exposed to charcoal-filtered air(<10 μg·kg-1) and elevated O3(80 μg·kg-1) after anthesis,aimed to investigate the responses of soybean’s agronomic characters,leaf area,chlorophyll content,antioxidant system,and grain yield to elevated O3.Under elevated O3,the leaf area and chlorophyll content decreased significantly(P<0.05),and the leaf catalase(CAT),superoxide dismutase(SOD),and peroxidase(POD) activities had a significant increase(P<0.01) first but a gradual decrease then,compared with those under charcoal-filtered air.Elevated O3 decreased the leaf soluble protein and ascorbate content(AsA) contents while increased the leaf malonaldehyde(MDA) content(P<0.05),suggesting that the leaf membrane lipid peroxidation was accelerated.The dry mass per plant,effective pod number,grain number,100-grains weight,and grain yield under elevated O3 had somewhat decreased,among which,grain yield decreased significantly,with the decrement reached to 47%(P<0.01).更多还原