【摘要】 在正常株高油菜中克隆了与拟南芥矮化突变相关基因AtGID1同源的BnGID1基因全长cDNA目的片段,将其正向插入表达载体pFGC5941上的CaMV35S启动子下游,构成该基因过量表达载体pFGC5941-BnGID1.经根癌农杆菌介导,导入甘蓝型油菜矮化突变体NDF-1基因组,通过PCR及半定量RT-PCR检测,确定得到抗性转基因植株23株.移至大田生长,观察结果显示,转基因植株明显高于对照矮化突变体NDF-1,平均高出49.05cm.结果表明,BnGID1基因过量表达能够使甘蓝型油菜矮化突变体NDF-1株高得到较大程度的恢复.图7表2参16更多还原

【Abstract】 A full-length cDNA of BnGID1 gene with 1 041 bp was cloned from Brassica napus L. BnGID1 is a homolog of Arabidopsis AtGID1 which was confirmed to encode a receptor protein of gibberellin. For addressing the function of BnGID1 gene, an over-expression binary vector pFGC5941-BnGID1 was constructed on basis of the binary plasmid pFGC5941 in which BnGID1 was driven by CaMV35S promoter. The dwarf ‘NDF-1’ plants were transformed via Agrobacterium tumefaciens containing pFGC5941-BnGID1. Twenty-three transgenic plants were then identified by PCR and semi-quantification RT-PCR. Grown in field, the heights of the transgenic plants and dwarf mutants were measured, respectively. The results showed that the transgenic plants were significantly higher than the plants of dwarf mutant NDF-1 with an average height of 49.05 cm, indicating that the BnGID1 gene over-expression could partially recover plant height of dwarf mutant NDF-1. Fig 7, Tab 2, Ref 16更多还原