【摘要】 目的:研究爬山虎中黄酮的鉴别及总黄酮的提取工艺。方法:采用传统溶剂浸提法进行常规的化学定性分析确定爬山虎籽中含有黄酮类化合物,并采用五因素四水平的正交试验优化提取工艺。结果:实验证实爬山虎中含有大量黄酮类化合物。正交实验得出了最佳提取工艺条件:提取温度为50℃,料液比为1∶12,提取时间为3h,提取剂为pH=2的65%乙醇溶液。以芦丁为标准品,用紫外分光光度法测定爬山虎籽中总黄酮含量为2.3%。结论:爬山虎籽中含有丰富的黄酮类化合物,优化并建立了爬山虎籽中总黄酮的提取工艺条件。更多还原

【Abstract】 Objective:To make a comprehensive study on identification and extraction technology of flavonoids from the parthenocissus seeds.Methods:Traditional solvent extraction method and orthogonal experiments were used.Flavonoids in the parthenocissus seeds were confirmed by conventional qualitative analysis.Results:The research showed that the parthenocissus seeds contain flavonoids.The optimum technical condition were summed up as follows through the orthogonal experiments:extraction temperature was at 50℃,proportion material and extracting reagent was 1 :12,the extracting time was 3 h,extracting reagent was 65% aqueous ethanol solution,and pH=2.Comparing to ruitin standard,extraction rate of flavonids in parthenocissus seeds was 2.3% by UV spectrophotometry.Conclusion:Flavonoids is rich in the parthenocissus seeds.The preliminary extracting technology of flavonoids from the parthenocissus seeds is established in the study.更多还原