【摘要】 目的:观察雷公藤内酯醇(Tri)对实验性自身免疫性脑脊髓炎(EAE)小鼠模型临床症状和脊髓组织IL-10、IFN-γ的影响。方法:C57BL/6黑色小鼠随机分为EAE组、Tri治疗组及佐剂组,采用MOG35～55免疫动物建立EAE模型,治疗组腹腔注射Tri治疗,观察和评定小鼠临床症状,免疫组化染色观察脊髓中IL-10、IFN-γ的表达,并行相关图像分析。结果:EAE组与Tri治疗组发病时间、发病率等均有显著差异;与佐剂组比较,EAE组IFN-γ表达明显增高(P<0.01),IL-10表达降低(P<0.05);与EAE组比较,Tri治疗组IFN-γ表达降低(P<0.01),IL-10表达明显增加(P<0.05)。结论:Tri可明显改善EAE小鼠临床症状,其可能机制之一是通过抑制Th1及其分泌的IFN-γ,促进Th2及IL-10的分泌,调整Th1/Th2以治疗EAE。更多还原

【Abstract】 Objective:To observe the effects of Triptolide (Tri) on IL-10 and IFN-γ in spinal cord of EAE mice. Methods:C57BL/6 mice were randomly divided into EAE group,Tri treatment group and adjuvant group. EAE model was established with MOG35-55,Tri was intraperitoneally injected in treatment group. The symptoms of mice in EAE were monitored according to the international standard of 5-scores-system. The spinal cord was used for IL-10,IFN-γ immunohistochemical staining. Results:The differences of incidence,change of body weight and mean maximum clinical scores between EAE group and Tri treatment group were statistically significant (P<0.05). After the intervention of Tri,compared with adjuvant group,the level of IFN-γ in EAE group was increased (P<0.01),and the level of IL-10 was decreased (P<0.05). Compared with EAE group,IFN-γ in Tri group was decreased (P<0.01),and the level of IL-10 was significantly increased (P<0.05). Conclusion:Tri can treat EAE mice effectively. It may be caused by depressing IFN-γ secreted by Th1 and promoting IL-10 secreted by Th2,which can regulate Th1/Th2.更多还原