【摘要】 目的对90份临床检测为丙型肝炎病毒抗体(HCV-Ab)阳性样品检测其核心抗原(HCV-cAg)、荧光定量PCR,观察3者间的相关性。方法采集临床诊断为HCV-Ab阳性样品90份,分别用市售的HCV-cAg检测试剂盒、荧光定量PCR检测试剂盒、HCV-Ab检测试剂盒进行3种指标的检测复核。结果 90份临床HCV-Ab阳性样品,检出HCV-cAg阳性25份(27.8%),荧光定量PCR检出67份阳性(74.4%),抗体复核阳性的75份(83.33%);检出率HCV-Ab(83.33%)>荧光定量(74.4%)>HCV-cAg(27.8%)。结论丙肝的3种标志物之间存在一定的联系,但不同试剂的检测结果间存在明显差异。更多还原

【Abstract】 Objective Clinical samples of 90 confirmed HCV-Ab positive cases were detected for core antigen(HCV-cAg) and HCV RNA by fluorescence quantitative PCR(FQ-PCR),and the relativity of the three was observed.Methods 90 samples diagnosed as HCV-Ab positive cases were collected,and the three indicators was detected and rechecked with HCV-cAg ELISA kit,HCV-Ab ELISA Kit and quantitative hepatitis C virus(HCV) PCR Fluorescence Diagnostic kit for sale in market separately.Results Among 90 clinical samples of HCV-Ab positive cases,HCV-cAg was positive in 25 cases(27.8%),FQ-PCR was positive in 67 cases(74.4%),and antibody rechecking was positive in 75 cases(83.33%).As regards to the positive rate,HCV-Ab was the highest and florescence quantitative took the second place,and HCV-cAg was the lowest.Conclusion It had some relations among the three makers of HCV,but there were significant differences in detection results of different agents.更多还原