【摘要】 目的评估白介素-11(Interleukin-11,IL-11)对小鼠异基因骨髓移植(allo-BMT)后急性移植物抗宿主病(aGVHD)的预防作用。方法用主要组织相容性抗原(MHC)完全不合的纯种近交系SPF级小鼠,雄C57BL/6(H-2b)为供鼠,雌性BALB/C(H-2d)为受鼠,建立allo-BMT/aGVHD动物模型,随机分3组:模型对照组、IL-11组(剂量为500μg/(kg.d)、环孢菌素A(CsA)组(剂量为10mg/(kg.d),观察其对aGVHD的预防作用。结果 IL-11组、CsA组受鼠生存时间均长于模型对照组(P<0.01);IL-11组、CsA组受鼠aGVHD临床表现和病理改变均较模型对照组明显轻。IL-11组、CsA组之间平均生存时间比较无统计学差异(P=0.901)。结论 IL-11可有效预防小鼠allo-BMT后aGVHD,减轻其症状和病理损害程度,显著延长平均生存时间。更多还原

【Abstract】 Objective To evaluate the prophylactic effect of interleukin-11(IL-11) on acute graft-versus-host disease (aGVHD) in a murine allogeneic bone marrow transplantation (allo-BMT) model. Methods Acute GVHD was induced in male BALB/C(H-2d) mice by total body irradiation (TBI) followed by female allogeneic C57BL/6(H-2b)bone marrow and spleen cells transplantation. The experiment groups were designed as follows: control group, IL-11 ( 500μg/(kg·d) group, cyclosporine A ([CsA, 10mg/(kg·d)] group. The occurrence of aGVHD was assessed by signs of weight loss, diarrhea, ruffled fur, hunched posture and histologic changes of skin, liver and small intestines. Results The survival time of IL-11 group and CsA group were prolonged as compared with that of the control group (P<0.01). aGVHD signs and histologic changes of IL-11 group and CsA group were milder than those of the control group. Conclusion IL-11 can be effective in prevention for aGVHD after allo-BMT in a murine model.It can decrease the aGVHD signs and histologic changes,and markly prolong mean survival time.更多还原