【摘要】 目的研究慢性淋巴细胞白血病(CLL)13q14缺失的情况。方法运用位于13q14的序列特异性DNA探针RB1、D13S319、D13S25和间期荧光原位杂交(I-FISH)技术对26例初发的B细胞CLL患者进行染色体13q14的检测。结果 26例B-CLL中14例(53.8%)有13q14缺失,阳性细胞率为30.0%～90.0%,其中RB1缺失2例(7.7%),D13S319缺失11例(42.3%),D13S25缺失13(50.0%);RB1、D13S319、D13S25同时缺失2例,D13S319、D13S25同时缺失有8例(30.7%)。结论 CLL患者13q14缺失区域是不恒定的。更多还原

【Abstract】 Objective To investigate the incidence of 13q14 deletion [del(13q14)] in chronic lymphocytic leukemia(CLL).Methods Fluorescence in situ hybridization(FISH) and sequence-specific DNA probes RB1,D13S319,D13S25 for 13q14 were applied to detect del(13q14) in 26 patients with B-CLL.Results Of the twenty-six patients,fourteen had 13q14 deletion,the incidence of del(13q14) was 53.8%.Among the patients,two had del(13q14) with RB1,eleven had del(13q14) with D13S319,thirteen had del(13q14) with D13S25,two had del(13q14) with RB1,D13S319 and D13S25,eight had del(13q14) with D13S319 and D13S25.Conclusion The region of loss at 13q14 is variable,the FISH is a rapid and sensitive technique for analysis of del(13q14) in CLL.更多还原