【摘要】 为了研究骨碎补的化学成分,采用硅胶、ODS、Sephadex LH-20柱色谱及半制备高效液相色谱等技术,从中药骨碎补的70%乙醇提取物中分离得到9个酚酸类化合物。其结构经1HNMR、13CNMR、2DNMR、HR-ESI-MS等谱学方法分别鉴定为4,4’-dihydroxy-3,3’-imino-di-benzoic acid(1)、原儿茶酸(protocatechuic acid,2)、没食子酸(gallic acid,3)、对羟基苯甲酸(p-hydroxybenzoic acid,4)、咖啡酸[(E)-caffeic acid,5]、ethyl trans-3,4-dihydroxycinnamate(6)、咖啡酸4-O-β-D-葡萄糖苷(caffeic acid 4-O-β-D-glucopyranoside,7)、对-香豆酸4-O-β-D-葡萄糖苷(p-coumaric acid 4-O-β-D-glucopyranoside,8)和23(S)-12-O-caffeoyl-12-hydroxyllauric acid glycerol ester(9)。其中,化合物1和9为新化合物,化合物3、4、6为首次从槲蕨属植物中分离得到。更多还原

【Abstract】 To study the chemical constituents of Drynariae Rhizoma,nine phenolic acids were isolated from a 70% ethanol extract by using a combination of various chromatographic techniques including column chromatography over silica gel,ODS,Sephadex LH-20,and semi-preparative HPLC.By spectroscopic techniques including 1H NMR,13C NMR,2D NMR,and HR-ESI-MS,these compounds were identified as 4,4’-dihydroxy-3,3’-imino-di-benzoic acid(1),protocatechuic acid(2),gallic acid(3),p-hydroxybenzoic acid(4),(E)-caffeic acid(5),ethyl trans-3,4-dihydroxycinnamate(6),caffeic acid 4-O-β-D-glucopyranoside(7),p-coumaric acid 4-O-β-D-glucopyranoside(8),and 23(S)-12-O-caffeoyl-12-hydroxyllauric acid glycerol ester(9),separately.Among them,1 and 9 are new compounds,and 3,4,and 6 were isolated from Drynaria species for the first time.更多还原