【摘要】 目的探讨准分子激光原位角膜磨镶术(laser in situ keratomileusis,LASIK)前后患者泪液中转化生长因子α(transforming growth factor-α,TGF-α)的浓度变化及其对角膜创伤愈合的作用。方法LASIK术前及术后1、3、7、15 d,采用双抗体夹心酶联免疫吸附法检测30例(60眼)患者泪液中TGF-α含量,对不同时间泪液中细胞因子平均浓度进行统计分析。结果LASIK术前及术后半个月随访期间,TGF-α在泪液中的浓度从术前(175.19±61.22)pg/mL升至术后7 d(243.27±75.51)pg/mL,差异有统计学意义(P<0.05),术后15 d(191.63±20.51)pg/mL,差异无统计学意义(P>0.05)。结论LASIK术后TGF-α表达比术前显著增加,促进角膜角膜细胞分裂、趋化及移行,有利于伤口愈合。更多还原

【Abstract】 Objective To quantify the concentration of transforming growth factor-α(TGF-α) in tears before and after laser in situ keratomileusis(LASIK) and to investigate the effect of TGF-α on wound healing of cornea.Methods LASIK was performed on 60 eyes of 30 patients,and the tears specimen was collected before LASIK and on day 1,3,7 and 15 after operation.The enzyme linked imunasorbent assay was used to quantitatively measure the concentration of cytokines in the tears.Results The expression of TGF-α increased significantly from(175.19±61.22) pg/mL preoperatively to(243.27±75.51) pg/mL on day 7 after operation(P<0.05),while to(191.63±20.51) pg/mL on day 15 after operation(P>0.05).Conclusions The increased expression of TGF-α may accelerate wound healing by the stimulation of cell preliferation and migration.更多还原