【摘要】 目的:通过体外细胞实验,探讨扶正抑瘤方有效治疗前列腺癌的作用机理。方法:以人前列腺癌PC-3细胞为模型,观察扶正抑瘤方高、中、低剂量(9.58、19.17、38.33 g/kg)及不同浓度含药血清对PC-3细胞形态、细胞增殖、细胞周期及细胞凋亡的影响。结果:扶正抑瘤方含药血清处理后,PC-3细胞可发现不同程度的细胞皱缩,染色质凝集,或细胞核固缩碎裂;MTT实验表明含药血清能明显抑制PC-3细胞增殖,且剂量越高细胞增殖抑制率越高,20%高剂量含药血清组抑制率约50%;流式细胞仪检测发现,含药血清组处理后48 h,各组在非凋亡细胞二倍体峰(G1)之前均出现大小不等的凋亡细胞峰,凋亡率均显著高于空白血清组,而且G0/G1期细胞比例增加,S期及G2/M期细胞比例下降。结论:扶正抑瘤方含药血清能抑制PC-3细胞增殖,且呈剂量、浓度依赖性,其抗肿瘤活性可能与诱导肿瘤细胞凋亡有关。更多还原

【Abstract】 AIM:To observe the effective mechanism of Fuzhengyiliu Fang(FZF) in the treatment of prostate cancer in vitro. METHODS:Serum of high,medium,low doses of FZF and control groups were prepared by serum pharmacology methods,human prostate cancer PC-3 cells applied as an experiment model,this study observed morphology,inhibition rate of cell proliferation,cell cycle and cell apoptosis of PC-3 cells affected by different doses and concentrations of FZF serum.RESULTS:After treated with FZF serum,PC-3 cells were found in different degrees of cell shrinkage,chromatin condensation,or nuclear condensation and fragmentation;MTT experiment showed FZF serum obviously restrained cell proliferation,and the higher the concentration,the higher the rate of inhibition,20% high-dose FZF containing serum group inhibited cell proliferation about 50%;flow cytometry detected that after treated 48 h,different sizes of the apoptotic peak had appeared before the diploid peak(G1) of non-apoptotic PC-3 cells,and the apoptosis rate of all treated groups were significantly higher than the control group,the proportion of G0/G1 phase cells increased,S and G2/M phase cells decreased.CONCLUSION:FZF serum can inhibit proliferation of PC-3 cells in dose,concentration-dependent trend,this activity may be related with its effect of inducing tumor cell apoptosis,which provide an important experimental basis for the clinical treatment of prostate cancer with FZF.更多还原