【摘要】 目的:观察低、高剂量四妙散加味方(SMSJWF)是否调节人肾小管上皮细胞(HK-2细胞)尿酸盐转运子(hUAT)mRNA和有机阴离子转运体(hURAT1)mRNA的表达。方法:根据培养液中所含四妙散加味方剂量的不同,将HK-2细胞分为:DMEM/F-12+6 mL/2 kg四妙散加味方低剂量组;DMEM/F-12+12 mL/2 kg四妙散加味方高剂量组;DMEM/F-12+苯溴马隆4.8 mg/d阳性对照组;仅使用DMEM/F-12+空白血清对照组。四组细胞分别培养48 h,采用实时荧光定量PCR检测HK-2细胞中hUATmRNA、hURAT1 mRNA的相对表达量(2△△Ct法)。结果:所有标本均能检测到hUAT mRNA和hURAT1 mRNA的表达。低、高剂量组和阳性对照组hUAT mRNA表达水平均明显高于空白对照组(P<0.05);低剂量和阳性对照组的hURAT1 mRNA的表达明显低于空白对照组(P<0.05),而高剂量组hURAT1 mRNA的表达明显高于其他各组(P<0.05)。结论:低、高剂量四妙散加味方可上调hUAT mRNA表达;低剂量四妙散加味方与苯溴马隆均可下调hURAT1mRNA的表达,这可能是其降尿酸的作用机制之一。更多还原

【Abstract】 AIM:To observe the effect of SiMiaoSanJiaWeiFang(SMSJWF) in different concentrations on the expression of human UAT and URAT1 transporter genes in HK-2 cells. METHODS:HK-2 cells were divided into 4 groups as follows:The low dosage group(cultured with DMEM/F-12 and 6 mL/2 kg SMSJWF);the high dosage group(cultured with DMEM/F-12 and 12 mL/2 kg SMSJWF);the positive control group(cultured with DMEM/F-4.8 mg/d Benzbromarone);the blank control group(cultured with DMEM/F-12).In each group,6 bottles of the cells were cultured in vitro for 48 hours.Then the relative quantity of hUAT and hURAT1 mRNA in the HK-2 cells was detected by real-time fluorescent quantitative PCR assay.RESULTS:hUAT and hURAT1 mRNA could be detected in all samples.And the level of hUAT mRNA in the blank control group were significantly lower than those in the other groups(P<0.05).Compared with blank control group,the expressions of hURAT1 mRNA in low dosage group and Benzbromarone group were reduced clearly(P<0.05).By contrast,the expression in high dosage group was increased clearly(P<0.05).CONCLUSION:SiMiaoSanJiaWeiFang up-regulates the expression of hUAT gene.SiMiaoSanJiaWeiFang down-regulates the expression of hURAT1 gene,which may be one of the mechanism to reduce uric acid levels.更多还原