【摘要】 目的:观察二氢杨梅素对血吸虫病肝纤维化的治疗作用。方法:将小鼠随机分为4组:正常对照组,模型组,吡喹酮组,吡喹酮+二氢杨梅素组。除正常对照组外,其余各组每只小鼠经腹部皮肤感染日本血吸虫尾蚴诱导肝纤维化模型,药物治疗组于感染8周后灌胃给予相应的药物。实验结束后,取小鼠血,测血清中ALT、AST;取小鼠肝脏称重,计算肝脏指数;观察小鼠肝脏形态;另取固定部位肝组织作病理组织学检查,测定肝脏Ⅰ和Ⅲ型胶原表达。结果:吡喹酮联合二氢杨梅素治疗较单纯吡喹酮治疗可降低血吸虫病肝纤维化小鼠肝脏指数及血清ALT、AST(P<0.05),改善肝组织形态结构,降低肝脏Ⅰ和Ⅲ型胶原表达(P<0.01)及肝纤维化病理评分(P<0.05)。结论:二氢杨梅素对血吸虫病肝纤维化有明显治疗作用。更多还原

【Abstract】 AIM:To observe the therapeutic effect of dihydromyricetin on experimental schistosoma japonicum hepatic fibrosis in mice. METHODS:60 mice infected with schistosma japonicum cercariae percutanoeusly were divided into 3 groups:model group,praziquantel group, praziquantel plus dihydromyricetin group and other 20 normal mice were used as control group.After treatment with medicine for 8 weeks,the liver was removed and weighed.The contents of ALT and AST in serum were assayed using the corresponding kits.Moreover, the degree of hepatic fibrosis was observed Via HE and was scored.The expression of collagenⅠprotein and collagenⅢprotein were measured by immunohistochernical method.RESULTS: The mice that infected with schistosoma japonicum, had a featuring increment in liver weights,serum ALT,AST contents,the expression of collagenⅠprotein,collagenⅢprotein (P<0.05).Praziquante treatment significantly reduced serum ALT,AST contents,the expres- sion of collagenⅠprotein,collagenⅢprotein(P<0.05).In addition the hepatic histopathology was improved in praziquantel plus dihydromyricetin group(P<0.05).The liver weights,serum ALT,AST contents in praziquantel plus dihydromyricetin group[(57.5±7.6) mg/g,(110±12) IU/L,(134±11) IU/L]were significantly lower(P<0.05) than those in praziquantel group[(50.5±5.2) mg/g,(87±13) IU/L, (110±11) IU/L].The expression of collagenⅠprotein,collagenⅢprotein in praziquantel plus dihydromyricetin group(0.1468±0.0232, 0.1305±0.0237) were significantly lower(P<0.01) than those in praziquantel group(0.2058±0.0216,0.1768±0.0224).CONCLUSION: Dihydromyricetin has significant therapeutic effect on schistosoma japonicum-induced liver fibrosis in mice.更多还原