【摘要】 目的:探讨脂联素基因SNP-11377(C/G)与中国汉族2型糖尿病(T2D)的关系及对罗格列酮降糖疗效的影响。方法:应用PCR-RFLP方法对255名T2D和120名健康对照者进行-11377(C/G)基因分型,选取42个不同基因型的T2D患者每天早饭时口服罗格列酮4mg,连续服药12周。测定用药前后的TG、空腹血糖(FPG)、餐后血糖(PPG)、糖化血红蛋白(HbAlc)、空腹胰岛素(FINS)、餐后胰岛素(PINS)、TC、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、胰岛素抵抗指数(HOMA-IR)和脂联素水平。结果:T2D组SNP-11377等位基因频率与基因型频率明显高于健康对照组(P<0.05),并且随着G等位基因的增加,患者的脂联素水平(P<0.01)明显降低,FINS(P<0.05)和HOMA-IR水平(P<0.05)明显增高。在服用罗格列酮后,CC型FPG、PPG和HOMA-IR的降低幅度明显高于突变型(CG+GG)(P<0.05),而CC型脂联素升高幅度明显高于突变型(P<0.01)。结论:脂联素基因启动子-11377C/G遗传变异与胰岛素抵抗和T2D有关,并且可能影响罗格列酮的降糖疗效。更多还原

【Abstract】 AIM:To evaluate the impact of adiponectin allele C-11377G genetic polymorphisms on efficacy of rosiglitazone in Chinese patients with type 2 diabetes(T2D).METHODS:Patients with T2D(n=255)and 120 healthy volunteers were enrolled to identify-11377C/G genotypes by polymerase chain reaction restriction fragment length polymorphism assay.Forty-two T2D patients with different-11377C/G genotypes received orally rosiglitazone as a single-dose therapy(4 mg day-1 p.o.)for 12 weeks.The serum triglyceride,fasting plasma glucose(FPG),postprandial plasma glucose(PPG),glycated hemoglobin(HbAlc),fasting serum insulin(FINS),postprandial serum insulin(PINS),total cholesterol(TC),low-density lipoprotein-cholesterol(LDL-C),high-density lipoprotein-cholesterol(HDL-C),homeostasis model assessment for insulin resistance(HOMA-IR),and adiponectin concentration were determined before and after rosiglitazone treatment.RESULTS:Compared with the health control group,the allele frequency of-11377(C/G)and the genotypic frequency were increased in T2D group(P<0.05),and as the G allele gene increased,the adiponectin level was significantly decreased,and the levels of FINS and HOMA-IR were significantly increased in T2D patients(P<0.05).After treated with rosiglitazone,the degree of the decrease of the FPG(P<0.01),PPG(P<0.05)and HOMA-IR(P<0.05)levels in the CC genotype was significant higher than that in the other genotypes.However,the degree of the increase of the serum adiponectin concentration(P<0.01)in the CC genotype was significantly higher than that in the other genotypes.CONCLUSION:These data suggest that the adiponectin allele-11377C/G polymorphisms significantly associated with the pathogenesis of T2D,insulin resistance,and the therapeutic efficacy of rosiglitazone in Chinese patients with T2D.更多还原