【摘要】 目的探讨异硫氰酸苯乙酯(PHI)逆转K562/A02细胞株阿霉素(ADM)耐药的可能机制。方法将耐ADM的人慢性粒细胞白血病K562/A02细胞株分别与不同浓度(0,5,10,20,30,40和50μM)的PHI共同孵育后,流式细胞术检测细胞周期、细胞内ADM浓度以及P-糖蛋白1(P-gp1)表达水平;逆转录聚合酶链反应(RT-PCR)检测PHI作用前后细胞内mdrl mRNA的转录水平。结果与对照组相比,联合作用48h后,随着PHI浓度的增加G2期细胞逐渐减少,G1期细胞明显增多,而S期细胞无明显变化;K562/A02细胞内ADM平均荧光强度均高于对照组;K562/A02细胞mdrl mRNA表达下降和P-gp1表达下调(P<0.05)。结论 PHI逆转K562/A02细胞耐药机制可能与细胞G1期阻滞和P-gp1表达下调有关。更多还原

【Abstract】 Objective To elucidate the mechanism of phenylhexyl isothiocyanate (PHI) on the reversal of K562/A02 cell line.Methods After adriamycin(ADM) resistant K562/A02 cells were incubated with different concentrations of PHI(5,10,20,30,40 and 50 μM) or without PHI (control),flow cytometry(FCM)was used to evaluate cell cycle distributions,intracellular concentration of ADM,and the expression of P-gp1,respectively.Furthermore,mdrl mRNA was detected by reverse transcription polymerase chain reaction(RT-PCR) assay before and after use of PHI.Results The number of K562/A02 cells of G2 phase decreased and G1 phase increased,while the S phase had no obvious changes.The intracellular concentrations of ADM in K562/A02 cells were all higher than those in control groups.The transcription of mdrl mRNA and expression of P-gp1 were decreased and down-regulated with the increase of PHI,respectively (P<0.05) when compared to those of control groups.Conclusion Multidrug resistance of K562/A02 can be partly reversed by PHI,which may be related to the arrest of G1 and the decrease of P-gp1 expression.更多还原