【摘要】 目的观察血管内皮生长因子C(VEGF-C)在胆管癌组织中的表达。方法采用逆转录-PCR和免疫组化技术检测VEGF-C蛋白和VEGF-CmRNA在胆管癌和非肿瘤胆管粘膜组织中的表达。结果胆管癌VEGF-C的阳性表达率明显高于非肿瘤胆管粘膜组织(84.1%vs.16.7%)(P<0.05)。胆管癌组中,有淋巴结转移者的VEGF-CmRNA表达明显高于无淋巴结转移者(0.24±0.03vs.0.17±0.05)(P<0.05)。生存分析表明,VEGF-C阴性表达患者的生存时间明显长于阳性表达患者。结论 VEGF-C可能对胆管癌的淋巴转移起促进作用。更多还原

【Abstract】 Objective To evaluate the expression and significance of vascular endothelial growth factor-C(VEGF-C) in cholangiocarcinoma.Methods The expressions of VEGF-C and its mRNA were detected by RT-PCR and immunohistochemistry in cholangiocarcinoma and non-tumor bile duct epithelium.Their relationship to clinicopathological parameters was analyzed.Results The positive expression rate of VEGF-C in cholangiocarcinoma was higher than that in non-tumor bile duct epithelium(84.1% vs.16.7%)(P<0.05).The expression of VEGF-C mRNA in cholangiocarcinoma with lymph node metastasis was higher than that without(0.24±0.03 vs.0.17±0.05)(P<0.05).The patients with VEGF-C negative expression survived longer than those with VEGF-C positive expression.Conclusion VEGF-C might promote lymphatic metastasis of cholangiocarcinoma.更多还原