【摘要】 目的观察在豚鼠肠缺血/再灌注损伤(I/R)过程中,银杏内酯B(GB)对豚鼠离体腹腔神经节(CG)神经元的保护作用。方法 30只豚鼠随机分为三组,正常组、I/R组和I/R+GB组(2.5×10-4mol/L)。建立豚鼠肠I/R模型,通过细胞内生物电记录和应用HE染色技术观察GB对CG神经元的保护作用。结果模型组动物CG神经元损伤。胞内记录表明GB可不同程度地改善并恢复CG神经元的生理功能,使细胞膜电位趋于稳定;给予去极化方波刺激后,在正常组有70.1%(61/87)细胞产生动作电位,I/R组仅有12%(3/25)产生动作电位,I/R+GB组有42.4%(20/48)细胞发生去极化,31.2%(15/48)细胞产生动作电位(P<0.05)。HE染色观察,在灌流GB后,CG神经元数量明显多于I/R组(P<0.05)。结论银杏内酯B在肠缺血/再灌注损伤中对豚鼠离体CG神经元具有一定的保护作用。更多还原

【Abstract】 Objective To study the protective effects of Ginkgolide B(GB) on celiac ganglion(CG) neurons induced by intestinal ischemia/reperfusion in guinea pig in vitro.Methods 30 guinea pigs were randomly divided into three groups: control group,ischemia/reperfusion(I/R) group,I/R+GB group(treated with GB 2.5×10-4 mol/L).The intestinal injury was induced by clamping the superiormesenteric artery for 30 min and reperfusion for 60 min.The intracellular bioelectric recording technique and HE staining were applied to monitor the protective effects of GB on celiac ganglion neurons.Results In this study,CG neurons of the ischemia/reperfusion group were injuried. The intracellular bioelectric recordings showed GB could improve and recovery some physiologic function of CG neurons,stabilized the potential of membrane.With the stimulation of depolarized square-wave,the action potential was observed in 70.1% (61/87) cells of normal group compared with 12%(3/25) cells of I/R group.Under the same condition,in I/R+GB group,42.4% (20/48) cells depolarized and the action potential was found in 31.2%(15/48) cells(P<0.05).In HE staining observation,after the perfusion with GB,the amount of CG neurons of the group was much more than that of I/R group (P<0.05).Conclusion GB is responsible for the protective effect on CG neurons which are induced by intestinal ischemia/reperfusion in guinea pigs in vitro.更多还原