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血管内皮生长因子在围植入期小鼠子宫内膜中的表达

Expression of vascual endothelial growth factor in the peri-implanation mouse endometrium

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【作者】 高晓妹陈晓蓉张荣宜

【Author】 Gao Xiaomei1,2,Chen Xiaorong1,Zhang Rongyi1(1Dept of Histology and Embryology,2First Clinical College,Anhui Medical University,Hefei 230032)

【机构】 安徽医科大学组织学与胚胎学教研室安徽医科大学第一临床学院

【摘要】 目的观察血管内皮生长因子(VEGF)在围植入期小鼠子宫内膜的表达分布状况和规律。方法应用免疫组化SP法检测妊娠3~6d昆明小鼠围植入期子宫内膜VEGF的表达情况,并以动情期未孕小鼠子宫做对照。结果在小鼠子宫内膜中可检测到VEGF的表达,其表达具有时间特异性和细胞特异性。孕3d,VEGF在子宫内膜的腔上皮和基质细胞中呈弱表达;孕4~5d,VEGF在基质细胞和腔上皮上表达最强;孕6d,VEGF表达较强,但主要表达于蜕膜细胞。结论围植入期小鼠子宫内膜持续表达VEGF,且在植入前后表达最强,提示VEGF可能在小鼠胚胎的植入过程中发挥重要作用。

【Abstract】 Objective To study the expression and distribution of vascular endothelial growth factor(VEGF)and its rule in the peri-implantation mouse endometrium.Methods Immunohistochemical SP staining method was used to detected the expression of VEGF in mouse endometrium on day 3~6 of pregnancy and the unpregnant mouse endometrium(estrous stage)were selected as controls.Results Immunoreactive VEGF was detected throughout the mouse endometrial tissue,and the expression was in time-specific and cell-specific.During the pregnancy of day 3,the expression was detected weakly in luminal epithelium and endometrial stromal cells.Day 4~5,VEGF in stromal cells and luminal epithelium expressed the strongest.On day 6,VEGF expression was stronger,but mainly expressed in decidua cells.Conclusion VEGF express continuously in mouse endometrium during day 3~6 of pregnancy,and the strongest expression is before and after the implantation.It suggests that VEGF might play an key role in the process of the mouse embryo implantation.

【基金】 安徽省人事厅重点基金(编号:KJ2007A034)
  • 【文献出处】 安徽医科大学学报 ,Acta Universitatis Medicinalis Anhui , 编辑部邮箱 ,2010年01期
  • 【分类号】R714
  • 【被引频次】16
  • 【下载频次】137
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