【摘要】 目的:探讨基泰对慢性乙型肝炎(简称慢乙肝)患者(chronic hepatitis B,CHB)树突状细胞(dentritic cell,DC)生物学性状的影响。方法:分离慢乙肝患者外周血单核细胞,在含GM-CSF/IL-4及不同浓度的基泰培养条件下制备DC。观察DC形态学变化,流式细胞仪(FCM)测其细胞表型分子CD1a,CD83,CD80及HLA-DR的表达,MTT法测定DC刺激同种异体淋巴细胞增殖能力。结果:与对照组相比,实验组倒置显微镜观察显示出典型的DC形态。基泰(100μg/mL)处理组的DC膜表面分子CD1a及CD80表达均增高(P<0.05);刺激同种异体淋巴细胞增殖能力(SI)增强(P<0.05)。结论:基泰一定程度上可改善CHB的DC免疫学活性。更多还原

【Abstract】 Objective:To investigate the effects of Jitai on the function of dendritic cells derived from patients with chronic hepatitis B(CHB).Methods:Dendritic cell(DC) derived from monocyte of the patients with chronic HBV infection and cultured with IL-4 /GMCSF were treated with different Jitai concentrations on 4 d.Cell morphology was monitored under light microscopy.Cell surface molecules including HLA-DR,CD80,CD83,CD1a were assayed by flow cytometry.T cell proliferation induced by DC was assayed by methyl thiazolyl tetrazolium(MTT).Results:The stimulatory capacity of Jitai(100 ug/mL) in the allogeneic mixed leukocyte reaction(All MLR) markedly enhanced compared with the control group(P<0.05).The expressions of CD1a on DC treated with Jitai was significantly higher than those of DC untreated with Jitai(P<0.05),so was the expression of CD80(P<0.05).Conclusion:Jitai in certain concentration can improve the immunology function of DC derived from CHB.更多还原