【摘要】 本研究旨在观察和比较Allo-HSCT后发生GVHD的患者血浆中趋化因子含量的变化,并分析其与GVHD发生的关系,以探索对GVHD具有诊断意义的生物标志物。从26名allo-HSCT患者抽取26份血样用于研究,其中发生aGVHD的患者血浆13份作为研究组,未发生aGVHD的患者血浆13份作为对照组,比较了两组患者的临床特点;用ELISA蛋白芯片法测定了这些样本中40个趋化因子的含量,采用SAM分析、聚类分析和t检验比较了两组间各因子的含量的变化。结果发现,HCC-1、MIF、IP-10、ITAC、TARC和NAP-2水平在两组间存在显著性差异,其中MIF在移植后患者的血浆中的含量极高,而TARC在两组间的差别达10倍以上。结论:上述趋化因子血浆浓度在移植前后的变化可作为判断aGVHD是否存在的依据,但它们确切的病理生理意义有待进一步研究。更多还原

【Abstract】 The objective of this study was to analyze the relationship between some chemokines and the pathogenesis of GVHD and to find some biomarkers with diagnostic significance through observing and comparing the changes of some chemokine levels in samples from patients with or without aGVHD after allogeneic hematopoietic stem cell transplantation(allo-HSCT).26 plasma samples were obtained from 26 patients undergoing allo-HSCT at various time points after transplantation,of which 13 samples from patients with aGVHD were served as investigating group and 13 samples from patients without GVHD after Allo-HSCT were used as control group.The patient characteristics between the two groups were compared,the levels of 40 chemokines in these samples were detected by ELISA,the changs of chemokine levels in samples of 2 groups were analyzed by means of significance analysis micoroarray(SAM),clustering method and c-test.The results showed that there were significant differences in levels of 6 chemokines including HCC-1,MIF,IP-10,ITAC,TARC and NAP-2 between 2 groups,in which the level of MIF in plasma samples after HSCT was the highest,the difference of TARC level between 2 groups was over 10-fold.It is concluded that the level changes of chemokines mentioned above can be used as a indicator of GVHD presence,but their pathogenetic role in occurence of aGVHD remains to be determined.更多还原