【摘要】 本文采用脂多糖处理的RAW 264.7细胞为炎症细胞模型,采用ELISA、实时荧光定量PCR、免疫印迹、免疫细胞化学等方法探讨了覆盆子提取物对炎症发生过程中促炎、抗炎细胞因子及NF-κB的影响。结果表明,覆盆子提取物能够明显抑制脂多糖处理的RAW 264.7细胞中IL-1β、IL-6、NO、TNF-α和COX-2的表达(P<0.01或P<0.05),增强IL-10、HO-1等抗炎细胞因子的表达(p<0.01或P<0.05),并且提取物能够抑制NF-κB的产生。因此,我们推断覆盆子提取物具有较好的抗炎作用,其起效的可能机制是通过抑制NF-κB激活来抑制促炎因子的产生,并促进抗炎因子的表达。更多还原

【Abstract】 Anti-inflammatory activity of Rubus idaeus L.and possible mechanisms involved were explored in lipopolysaccharide (LPS)-treated RAW 264.7 cells.The effects of ethanol extract of R.idaeus on the levels of pro-inflammatory cytokines,including tumor necrosis factor-alfa(TNF-α),interleukin-6(IL-6) and interleukin-1 beta(IL-1β),as well as pro-inflammatory mediators, such as nitric oxide(NO),inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) were studied by Sandwich EL1SA,real-time PCR,and Western blot analysis.Moreover,the effects of ethanol extract of R.idaeus on anti-inflammatory cytokine interleukin-10(IL-10) and anti-inflammatory mediator heme oxygenase-1(HO-1) were also investigated using the same methods.Furthermore,nuclear factor-κB(NF-κB) level was assayed by immunocytochemistry.The results showed that the production of IL-1β,IL-6,NO,TNF-αand COX-2 in LPS-treated cells could be significantly inhibited(P<0.01 or P<0.05) by ethanol extract of R.idaeus compared with that in the cells treated with LPS only.Meanwhile,the production of NF-κB was also inhibited by the extract.Based on these results,the anti-inflammatory activity of R.idaeus was attributed to the down-regulation of IL-6,IL-1βand TNF-αlevels as well as gene expression of iNOS and COX-2 through the suppression of NF-κB activation, and induction of anti-inflammatory cytokine IL-10 and anti-inflammatory mediator HO-1.更多还原