【摘要】 目的研究建立12个mini-X-STR和Amel基因座复合扩增体系。方法选择DXS101、DXS10159、DXS10162、DXS10164、DXS6789、DXS7133、DXS7423、DXS7424、DXS8378、DXS981、GATA165B12和GA-TA31E08共12个X-STR与Amelogenin基因座,自行设计引物,分别采用FAM、HEX、Tamra、ROX四色荧光标记5′端,并进行必要的修饰,按照合适的引物浓度进行混合。对97个血痕样本进行复合扩增,并用ABI3130XL进行电泳和分型。结果所建立的荧光标记复合扩增体系能够得到清晰、明确的分型图谱,灵敏度达50pg,稳定性、重复性与平衡性较佳。结论本研究建立的12个mini-X-STR和Amel基因座荧光标记复合扩增体系统检验方法简单,灵敏度高,适合实际办案的需要。更多还原

【Abstract】 Objective To develop a 12 mini-X-chromosomal STR multiplex PCR system.Methods DNA were extracted from bloodstains.Primers of 12 X-chromosomal STRs(DXS7133,DXS8378,DXS981,DXS7424,DXS6789,DXS10159,GATA165B12,DXS101,DXS7423,DATA31E08,DXS10164,DXS10162) and Amelogenin gene were labeled with four different fluorescent dyes: FAM,HEX,TAMRA and Rox.PCR were performed in a single reaction.PCR products of 97 bloodstains samples were separated on Genetic analyzer 3130XL and STRs were typed by using GeneMapper ID 3.2.Results All of 97 samples have a clear and unambiguous typing,although mini-stutters were observed in a few loci.The same typing could be gained when samples were typed repeatedly.The sensitive of this multiplex PCR was low to 50pg.Conclusion Established multiplex PCR system for 12 mini-X-chromosomal STR loci and amelogenin gene can be used in forensic practice.更多还原