【摘要】 在酸性条件下,可溶性锰(Ⅳ)氧化D-异抗坏血酸产生微弱的化学发光,甲醛对该化学发光反应有明显的增敏作用.据此建立了快速、简便、灵敏的流动注射化学发光测定甲醛的新方法.本法测定甲醛的线性范围为0.05～6.0μg/mL,方法的检出限为0.03μg/mL,对2.0μg/mL的甲醛平行测定11次,其相对标准偏差为2.0%.本法用于水发火锅食品中甲醛的测定,与乙酰丙酮法测定结果基本一致,采用标准加入法,回收率为94.4%～101.2%,结果令人满意.更多还原

【Abstract】 It is found that D-isoascorbic acid can react with soluble manganese(Ⅳ)in the acidic medium and produce chemiluminescence,which is greatly enhanced by formaldehyde.The optimum conditions for this chemiluminescent reaction are in detail studied using a flow injection system.The experiments indicate that under optimum conditions,the chemiluminescence intensity is linearly related to the concentration of formaldehyde in the range 0.05-6.0μg/mL with a detection limit(3σ)of 0.03μg/mL.The relative standard deviation for the determination of 2.0μg/mL formaldehyde(n=11)was less than 2.0%.The proposed method was applied successfully to the determination of formaldehyde in hotpot food and the recovery is in the range of 94.4%-101.2%.The results were in good agreement with those obtained acetyacetone method.更多还原