【摘要】 采用荧光光谱法、紫外可见吸收光谱法研究了牛血清白蛋白(BSA)与水溶性ZnO量子点(QDS)的相互作用。结果显示,BSA使QDS的荧光增强并发生红移(从353 nm移至359 nm),说明量子点与蛋白间发生了相互作用;另一方面,QDS使BSA的荧光猝灭且最大发射峰发生明显蓝移(从336 nm移至326 nm),进一步说明量子点与蛋白间发生了相互作用。QDS与BSA作用后发射光谱的变化可能是由于二者之间存在配位及静电相互作用。QDS与BSA的相互作用表明QDS可以用来标记BSA。初步探讨了QDS对BSA的荧光猝灭机理。更多还原

【Abstract】 The interaction of bovine serum albumin(BSA) with water soluble ZnO quantum dots(QDS) was investigated by fluorescence spectroscopy.The fluorescence spectra shows that the addition of BSA results in the fluorescence intensity enhancement and red shift(from 353 nm to 359 nm) in the maximum emission peak of QDS,which indicates that there is interaction between BSA and QDS.On the other hand,the fluorescence of BSA quenched and blue-shifted(from 336 nm to 326 nm) in the presence of QDS,which further confirmed the interaction of BSA and QDS.The change in absorption spectrum and fluorescence spectrum was probably caused by the coordination and electrostatic interaction between QDS and BSA.The interaction of BSA with QDS indicates that QDS could label BSA.The mechanism has also been discussed according to fluorescence quenching effect of QDS on BSA.更多还原